摘要
[目的]为测定食品中的生物胺寻求一种快速、简单的方法。[方法]采用以丹磺酰氯作为衍生剂,室温离子液体作为萃取、富集和衍生介质,以超声作为反应条件的液相色谱法测定食品中的生物胺。[结果]测定食品中的生物胺较理想的衍生条件是:衍生剂量1.5ml,硼砂缓冲液的pH9.10,硼砂缓冲液量为2 ml,1 ml[OMIM]PF6离子液,超声时间为20 min。[结论]液相色谱法是测定食品中生物胺含量的一种快速、简单的方法。
[Objective]The aim was to look for a quick,easy way to determine biogenic amines in food.[Method]Using the dansyl chloride as a derivative agent,room temperature ionic liquids as extraction,enrichment and derivative media,as reaction conditions to ultrasonic liquid chromatography of biogenic amines in food.[Result]The result showed that determination of biogenic amines derived ideal conditions was that derived dose of 1.5 ml,borate buffer of pH 9.10,borate buffer volume of 2 ml,1 ml PF6 ionic liquid,ultrasonic time of 20 min.[Conclusion] Determination of liquid chromatography was the content of biogenic amines in a rapid and simple method.
出处
《安徽农业科学》
CAS
北大核心
2011年第14期8578-8581,共4页
Journal of Anhui Agricultural Sciences
关键词
离子液体
生物胺
高效液相色谱
Ionic liquids
Biogenic amines
High performance liquid chromatography
