摘要
目的:通过比较海康灵复方中单味药对过氧化氢(H2O2)诱导SH-SY5Y神经细胞损伤的保护作用,筛选更为理想的量效关系。方法:采用体外细胞培养的方法,复制神经母细胞瘤(SH-SY5Y)细胞H2O2损伤模型。通过MTT法测定细胞存活率,检测海康灵复方中单味药与复方对SH-SY5Y细胞损伤的保护作用。结果:根据最佳有效剂量组建的海康灵新复方(浓度为0.159g.mL-1)同单味药与原复方比较,能减轻H2O2引起的SH-SY5Y细胞的损伤,明显提高细胞的存活率。结论:海康灵新复方对H2O2诱导的SH-SY5Y细胞损伤具有明显的保护作用。
OBJECTIVE：To compare the protective effects of ingredients in the formula of Haikangling on SH-SY5Y cells injury induced by hydrogen peroxide so as to screen ideal dose-effect relationship.METHODS：SH-SY5Y cells injury induced by hydrogen peroxide was established by in vitro culture method.The protective effects of ingredients in the formula of Haikangling were observed by MTT assay.RESULTS：The new formula of Haikangling（0.159 g·mL-1） could relieve SH-SY5Y cells injury and increase the survival rate of the cells,compared with single ingredient and original formula.CONCLUSION：The new formula of Haikangling shows significant protective effect on SH-SY5Y cells injury induced by hydrogen peroxide.
出处
《中国药房》
CAS
CSCD
北大核心
2011年第23期2116-2118,共3页
China Pharmacy
基金
山东省中医药科技发展计划项目(2007-161)
关键词
海康灵
过氧化氢
神经母细胞瘤细胞
量效关系
Haikangling
Hydrogen peroxide
SH-SY5Y cells
Dose-effect relationship
