摘要
目的探讨别隐品碱(allocryptopine)对实验性肝纤维化的防治作用。方法采用CCl4复合因素诱导大鼠肝纤维化与血吸虫致小鼠肝纤维化模型,观察别隐品碱在造模同时(预防给药)和造模后(治疗给药)给药对脏器指数、肝功能、纤维化血清指标、脂质过氧化指标、肝组织胶原(CoI、CoIII)及羟脯氨酸(Hyp)表达的影响,并进行肝脏病理学检查。结果对CCl4所致肝纤维化大鼠模型,各剂量别隐品碱预防给药均能不同程度地降低肝指数及CoI表达(P<0.05、0.01),其中高剂量明显降低CoIII表达(P<0.01),中剂量明显降低脾指数、丙氨酸氨基转移酶(AST)的量(P<0.05);别隐品碱治疗组大鼠的肝指数、天冬氨酸氨基转移酶(ALT)的量、CoIII表达明显降低(P<0.05、0.01);预防给药与造模后给药均不同程度地减轻模型大鼠肝纤维化程度。对血吸虫致肝纤维化小鼠模型,别隐品碱预防给药显著降低小鼠ALT的量与Hyp水平(P<0.05),高剂量别隐品碱治疗给药能明显降低肝指数,降低血清PCIII、碘透明质酸(HA)、ALT的量(P<0.05、0.01),中剂量组肝指数、PCIII、HA的量明显降低(P<0.05);治疗给药组大鼠肝脏病变明显减轻。结论别隐品碱对实验性肝纤维化大鼠有较好的保护肝细胞、改善肝功能、抗肝纤维化作用。
Objective To investigate the prophylactic and therapeutic effects of allocryptopine on experimental hepatic fibrosis.Methods Experimental hepatic fibrosis models were induced by injection of tetrachloride in combination with the drinking of 5% alcohol to rats and Schistosoma japomicum infection to mice.The effects of allocryptopine anti-hepatic fibrosis were evaluated by comparing the liver and spleen indexes,tissue biochemical indices（ALT,AST）,lipid peroxidation indices（GSH-PX,MDA,SOD）,serum fibrosis indices（HA,PCIII LN）,the expression level of Hyp and collagen type I,III（CoI,CoIII）,and the liver pathology before and after allocryptopine intervention.Results In CCl4-induced liver fibrosis model rats,compared with model group,the liver index and the expression level of CoI were obviously decreased in allocryptopine prophylactic groups（P〈0.05,0.01）,high-dose prophylactic allocryptopine could significantly reduce the expression level of CoIII（P〈0.01）,middle-dose prophylactic allocryptopine could obviously reduce the spleen index,the content of AST（P〈0.05）;in allocryptopine therapeutic group,the liver index,the content of ALT,and the expression level of CoIII were significantly decreased（P〈0.05,0.01）.In S.japomicum-induced liver fibrosis model mice,prophylactic allocryptopine could reduce the content of ALT and the expression level of Hyp（P〈0.05）;the liver index,the contents of PCIII,HA,and ALT were significantly decreased in high-dose allocryptopine therapeutic group（P〈0.05,0.01）,the liver index,the contents of PCIII,and HA were obviously decreased in middle-dose allocryptopine therapeutic group（P〈0.05）;In addition,the hepatic histopathology was also improved in varying degrees after allocryptopine intervention.Conclusion Allocryptopine has certain effects on anti-injury for hepatocyte,ameliorating liver function,and prohibiting hepatic fibrosis.
出处
《中草药》
CAS
CSCD
北大核心
2011年第6期1158-1163,共6页
Chinese Traditional and Herbal Drugs
基金
科技部国际科技合作计划(2009DFA31270)
关键词
别隐品碱
肝纤维化
CCL4
血吸虫
脂质过氧化
allocryptopine
hepatic fibrosis
CCl4
Schistosomajapomicum
lipid peroxidation
