摘要
目的研究骨髓CD34＋细胞种植于人工血管后血清6-k-PGF1α和TXB2的变化情况，及其与新生内膜增生、血栓形成的关系。方法选杂种犬12条，分为ePTFE血管实验组（4条），涤纶血管实验组（4条）膨体聚四氟乙烯（ePTFE）对照组（2条）和涤纶对照组（2条），实验犬采自体骨髓，提取CD34＋细胞种植覆膜人工血管，对照犬采用单纯自体血预凝人工血管，经手术将血管植入同一条犬的下腔静脉和腹主动脉。在术前、术后3、7、14、30、60d经股静脉采血测定血小板，血清PGF1α、TXB2浓度和PGF1α／TXB2的比值，并在60d时获取全部人工血管标本，光学显微镜和电子显微镜观察新生内膜增生和血栓形成情况。结果实验组血小板及TXB2浓度先上升后下降并维持在一定水平，而对照组明显上升后维持在一个较高水平；实验组血清PGF1α及P／T先降低后升高，而对照组明显降低后维持在一个较低水平；对照组新生内膜较实验组明显增生，并有血栓形成。结论骨髓CD34＋干细胞种植人工血管能有效抑制PGF1α和P／T比值的过度降低、限制TXB2和血小板的过度升高，进一步抑制内膜的过度增生和血栓形成。
Objective To investigate the relationship between the 6-Keto-PGF1α, TXB2 and the endotheliosis and thrombosis of the new endomembrance on the prostheses seeded by CD34＋ stem cells. Method Twelve dogs were evenly divided into two groups： ePTFE prostheses and weaved Dacron prostheses implanted into the abdominal aorta （AA） and inferior vena cava （IVC）. in each group, 4 dogs were implanted by prostheses seeded by CD34＋ stem cells, 2 dogs were implanted prostheses treated by autogenous blood only as control. The peripheral blood was collected in preoperative and 3,7,14,30,60 days postoperation. Light and electron microscopy were applied to observe the endotheliosis and thrombosis of the new endomembrance when harvesting all the prostheses in the 60 days. Result The platelet, 6-Keto-PGF1α, TXB2 and P/T in experimental group was significantly different compared with control group. The new endomemhrane in control group was significantly thickener than the experimental group. Conclusions CD34＋ stem cell inhibits PGF1α and P/T ratio decrease, restrains TXB2 and platelet excessively rise, prevents intimal hyperplasia and thrombosis in a CD34＋ stem cells seeded blood vessel prostheses.
出处
《中华普通外科杂志》
CSCD
北大核心
2011年第5期421-425,共5页
Chinese Journal of General Surgery
基金
山东省自然科学基金资助项目（Z2004C02）
关键词
干细胞移植
人工血管
抗原CD34
前列环素
血栓素B2
Stem cell transplantation
Blood vessel prosthesis
Antigens, CD34
Prostacyclin
Thromboxane B2
