摘要
目的研究异常黑胆质成熟剂水提物总酚与顺铂、多西他赛联合对体外培养的人宫颈癌SiHa细胞增殖的影响,并探讨其作用机制。方法总酚分别与顺铂、多西他赛联合作用于SiHa细胞,采用MTT法检测细胞增殖抑制情况,倒置相差显微镜观察细胞形态,流式细胞术检测SiHa细胞Bcl-2、端粒酶表达水平。结果总酚-顺铂组、总酚-多西他赛组的细胞增殖抑制率分别明显高于顺铂组、多西他赛组(P<0.05),且细胞体积变小、变圆,可见较多脱落圆形死亡细胞,细胞Bcl-2、端粒酶表达水平显著降低(P<0.05)。结论异常黑胆质成熟剂总酚与顺铂、多西他赛联合可能通过降低Bcl-2和端粒酶表达水平而抑制SiHa细胞的增殖。
Objective To investigate the effects of combination of total phenolics from abnormal Savda Munziq water extracts with cisplatin or docetaxel on human cervical cancer SiHa cells in vitro,and to explore the mechanism.Methods SiHa cells were treated with total phenolics combined with cisplatin or docetaxel.Inhibition of cellular proliferation was detected by MTT assay,cellular morphology was observed by inverted phase microscope,and levels of Bcl-2 and telomerase expression in SiHa cells were detected by flow cytometry.Results The inhibitory rates of cellular proliferation of total phenolics-cisplatin group and phenolics-docetaxel group were significantly higher than those in cisplatin group and docetaxel group（P〈0.05）,respectively.Cells became small and round,and a lot of detached,round and dead cell could be seen.Levels of cellular Bcl-2,and telomerase expression were markedly decreased（P〈0.05）.Conclusion Total phenolics from abnormal Savda Munziq combined with cisplatin or docetaxel can inhibit proliferation of SiHa cells via inhibiting cellular Bcl-2 and telomerase expression levels.
出处
《重庆医学》
CAS
CSCD
北大核心
2011年第18期1769-1771,F0003,共4页
Chongqing medicine
基金
国家自然科学基金资助项目(30860325)
