摘要
目的检测肺结核患者血清中肺表面活性物质相关蛋白D(SP-D)的表达,探讨其在肺结核诊断中的意义。方法采用ELISA方法检测101例肺结核患者、30例慢性阻塞性肺疾病患者(COPD)、85例健康人血清中SP-D的水平并测定血清结核抗体,沉降法检测血沉,电阻法测定白细胞计数、中性粒细胞比例、淋巴细胞比例,芒图试验法进行结核菌素试验(PPD试验),抗酸染色检测痰中结核菌数量。结果肺结核患者血清中SP-D的水平明显高于健康人、低于COPD患者(P<0.05);ROC曲线显示肺结核患者血清SP-D的Cutoff值为0.26,在肺结核病诊断中敏感性为72.30%,特异性91.00%,诊断准确率为80.65%;PPD试验及结核抗体与血清SP-D具有相关性(2χ分别为8.44、17.08,P均<0.01)。结论血清SP-D与肺结核疾病具有重要的相关性,有望成为肺结核诊断的一种辅助检测指标。
Objective To investigate the change of serum surfactant protein-D（SP-D） in pulmonary tuberculosis patients,observe its relation with gender,age and other factors,and explore value of diagnosis for pulmonary tuberculosis.Methods The level of SP-D in the serum was detected by ELISA in 101 cases of pulmonary tuberculosis,30 cases of chronic obstructive pulmonary disease（COPD） and 85 healthy people.Leucocyte count,neutrophilic granulocyte percentage and lymphocyte percentage were detected with the electric resistance method,erythrocyte sedimentation rates by the sedimentation process,tubercle bacillus antibodies by ELISA,purified protein derivative（PPD） trial by the Mantoux method,and count of tubercle bacilli by acid-fast stain.Results The level of SP-D in the serum from pulmonary tuberculosis patients was significantly higher than that of the healthy,while it was significantly lower than that of COPD patients（P〈0.05）.The cutoff value of SP-D was 0.26 determined by the ROC curve drawn according to the measured values.The sensitivity,specificity and accuracy of detection of SP-D in the serum were 72.30%,91.00%,and 80.65% respectively in the diagnosis of pulmonary tuberculosis.There were no obvious differences in SP-D levels in different conditions of gender,age,leukocyte count,neutrophilic granulocyte percentage,lymphocyte percentage,sedimentation process and count of tubercle bacilli in the sputum and hollow in the lung,however,there were correlations of SP-D level with tubercle bacillus antibodies and PPD trial（values of were 8.44 and 17.08 respectively,P〈0.01）.Conclusions Detection of SP-D in serum is of great significance in the diagnosis of pulmonary tuberculosis,may become a new diagnostic method,and is worth exploring.
出处
《山东大学学报（医学版）》
CAS
北大核心
2011年第5期125-127,131,共4页
Journal of Shandong University:Health Sciences
基金
山东省科学技术厅科研基金资助项目(2006GG2202035)
