摘要
目的 探讨血清中佐匹克隆的测定方法。方法 样品在ｐＨ６－８６ 条件下用二氯甲烷一异戊醇（９８∶２） 提取，残渣用１－０×１０ －３ ｍｏｌ／Ｌ 盐酸溶解后测定溶液中佐匹克隆的荧光强度Ｆ（λｅｘ ＝３６５ ｎｍ 、λｅｍ ＝ ５２０ ｎｍ）。结果 血清中佐匹克隆的最低检出浓度为０－０３ ｍｇ／Ｌ，平均提取回收率为８４－０ ％ ，Ｒｓ 为３－７ ％ 。结论 本法可用于血清中佐匹克隆的测定。
Objective To determine the zopiclone concentration in serum. Methods The samples were adjusted to pH 6.86 and extracted with dichloromethane-isoamyl alcohol(98∶2). The remains were dissolved with 1.0 ×10 -3 mol/L HCl and the fluorescent strength of zopiclone (λ ex =365 nm,λ em =520 nm)was determined. Results The average recovery was 84.0% and R s was 3.7%.The limit of detection was 0.03 mg/L. Conclusion This method is suitable for determining zopiclone in serum.
出处
《山西医科大学学报》
CAS
1999年第4期378-380,共3页
Journal of Shanxi Medical University
