摘要
目的探讨去铁胺(DFO)对人成骨细胞株(hFOB1.19)骨相关基因Ⅰ型胶原(COL1)、骨保护素(OPG)和骨钙素(BGP)基因表达的影响。方法 hFOB 1.19在34℃下进行体外培养,培养基中加入不同浓度的DFO(5、10、20μmol/L)培养48 h后,收集细胞,分别抽取总RNA,采用半定量RT-PCR法检测hFOB 1.19中COL1、OPG、BGP mRNA的表达。结果 DFO浓度依赖性上调hFOB 1.19 COL1、OPG、BGP mRNA的表达(P<0.05)。结论 hFOB 1.19的COL1、OPG、BGP是hFOB 1.19活性相关基因;DFO对hFOB 1.19功能有促进作用,对防止骨质疏松症有一定潜在价值。
Objective To investigate the effects of deferoxamine（DFO） on the gene expressions of osteoprotegerin（OPG）,collagen-I（COL1） and osteocalcin（BGP） in human osteoblasts in vitro.Methods Osteoblasts were cultured at 34℃ in vitro.The different DFO concentrations（5,10,20μmol/L） were added into the culture medium.After treated with different concentrations of DFO for 48h,total RNA was isolated from osteoblasts.Expressions of COL1,OPG and BGP were detected by semiquantitative RT-PCR.Results DFO remarkably increased the expressions of COL1,OPG and BGP mRNA in human osteoblasts in a dose-dependent manner.Conclusion DFO upregulates the expressions of COL1,OPG and BGP in osteoblasts and has a potential role in preventing and treating osteoporosis.
出处
《江苏医药》
CAS
CSCD
北大核心
2011年第11期1255-1258,共4页
Jiangsu Medical Journal
基金
江苏省自然科学基金(BK2008165)
教育部博士点基金(20103201110020)
关键词
人成骨细胞
去铁胺
Human osteoblasts
Deferoxamine
