摘要
采用高效阴离子色谱-脉冲安培检测法（HAPEC-PAD）测定17种食用菌中海藻糖、甘露醇和阿糖醇含量。结果表明：阿糖醇、海藻糖和甘露醇的线性范围分别为239.62~1.25、59.41~0.15、148.29~5.00μg/mL,检出限分别为0.41、0.04、.11μg/mL,定量限分别为1.14、0.10和5.79μg/mL;17种食用菌中,香菇（Lentinula edodes）和猴头菌（Hericiumerinaceus）中阿糖醇含量较高,茶树菇（Agrocybe aegerita）、刺芹侧耳（Pleurotus eryngii）、鲍鱼侧耳（Pleurotus abalonus）、松口蘑（Tricholoma matsutake）、蛹虫草（Cordycepsmilitaris）中海藻糖含量较高,姬松茸（Agaricus blazei）、鸡油菌（Cantharellus cibarius）、金顶侧耳（Pleurotus citrinopileatus）、冬虫夏草（Cordyceps sinensis）中甘露醇含量较高;该项检测技术分离效果好、样品测定时不需要衍生化处理、检测时间短,是测定食用菌中功能性寡糖和糖醇类物质的有效方法。
A method for determining trehalose,mannitol and arabitol levels in the fruit bodies of 17 edible fungi using high performance anion chromatography-pulsed amperometric detection（HAPEC-PAD） is described.Samples could be tested without derivatization,and arabitol,trehalose and mannitol measurements were linear over the ranges 239.62-1.25,59.41-0.15 and 148.29-5.00 μg/mL,respectively.The limits of detection（LOD） for arabitol,trehalose and mannitol were 0.41,0.04 and 2.11μg/mL,respectively and the limits of quantification（LOQ） were 1.14,0.10 and 5.79 μg/mL,respectively.High levels of arabitol were recorded in Lentinula edodes and Hericium erinaceus,high levels of trehalose in Agrocybe aegerita,Pleurotus eryngii,Pleurotus abalonus,Tricholoma matsutake and Cordyceps militaris,and high levels of mannitol in Agaricus blazei,Cantharellus cibarius,Pleurotus citrinopileatus and Cordyceps sinensis.Our data demonstrate that HAPEC-PAD is an effective method for detecting these analytes in edible fungi,and provides a useful tool for research on their biological functions.
出处
《食用菌学报》
北大核心
2011年第1期49-52,共4页
Acta Edulis Fungi
基金
上海市科技兴农重点攻关项目[编号:沪农科攻字(2009)第2-2号]的部分研究内容
关键词
食用菌
高效阴离子色谱
脉冲安培检测器
海藻糖
甘露醇
阿糖醇
Edible fungi
high performance anion chromatography
pulsed amperometric detection
trehalose
mannitol
arabitol
