摘要
目的了解中国不同地区慢性乙型肝炎病毒(HBV)感染者中HBV基因型及基因亚型分布特点。方法从我国东部(山东、江苏)、西部(新疆)、南部(广东、广西)、北部(吉林、北京)及中部(河南、河北)5个地区的9个省、自治区、直辖市收集735份慢性HBV感染者血清样品,用本实验室建立的型特异性引物巢式聚合酶链反应法(nPCR)检测HBV基因型及基因亚型。结果 735份样品中,71.8%(528/735)为C型,10.3%(76/735)为B型,6.3%(46/735)为D型,11.6%(85/735)为混合型,未检测到其他基因型。528份C型样品中,C2亚型占94.0%(496/528),C1亚型占4.9%(26/528),C1+C2混合亚型占1.1%(6/528);76份B型样品中,B2亚型占90.8%(69/76),B1亚型占5.3%(4/76),B1+B2混合亚型占3.9%(3/76)。除西部新疆地区C型相对较少(31/116,26.7%)外,其他地区均以C型为主。D型主要分布在西部新疆地区,占31.9%(37/116)。混合型在各地区均有发现,但以西部和南部地区相对多见,分别占30.2%(35/116)和20.0%(22/110)。结论我国HBV基因型和基因亚型的分布有明显的地域性差异。除西部新疆地区以D型为主外,其他地区均以C型为主,且主要为C2亚型。B型中主要为B2亚型。
Objective To investigate the characteristics of the genotypes and subgenotypes distribution of hepatitis B virus（HBV） among chronic hepatitis B patients in the different areas of China.Methods A total of 735 serum samples of chronic hepatitis B patients were collected from nine provinces,which were located in the eastern（Shandong and Jiangsu）,western（Xinjiang autonomous region）,southern（Guangdong and Guangxi）,northern（Jilin and Beijing）,and central（Henan and Hebei） areas of China,respectively.HBV infection was verified by a nested polymerase chain reaction（nPCR） method for each collected specimen.The method of strain typing was established and applied for the detection of the genotypes A-D and subgenotypes B1,B2,C1 and C2 of HBV.Results Of 735 samples genotyped,71.8%（528/735） were genotype C,10.3%（76/735）genotype B,6.3%（46/735）genotype D,and 11.6%（85/735） mixed genotypes.None of any other genotypes was found.Among the 528 genotype C isolates,94.0%（496/528）,4.9%（26/528）and 1.1%（6/528） were subgenotype C2,C1 and C1＋C2 mixed strains,respectively.Among the 76 genotype B isolates,90.8%（69/76）,5.3%（4/76）and 3.9%（3/76）were subgenotype B2,B1 and B1＋B2 mixed strains,respectively.Genotype C was the dominant strain in China with the exception of Xinjiang Uygur Autonomous Region（31/116,26.7%） where genotype D was endemic（37/116,31.9%）.Mixed genotypes were confirmed in each of the areas,and more frequently detected in the west（35/116,30.2%） and the south（22/110,20.0%） of China.Conclusions The genotypes and subgenotypes of HBV have distinct geographic distribution among chronic hepatitis B patients in China.HBV genotype C is dominant in China with the exception of Xinjiang Uygur Autonomous Region where genotype D is prevalent instead.B2 and C2 are the main subgenotypes of genotypes B and C,respectively.
出处
《中国病毒病杂志》
CAS
2011年第3期177-182,共6页
Chinese Journal of Viral Diseases
基金
国家"十一五"艾滋病和病毒性肝炎等重大传染病防治科技重大专项(2008ZX10002-001)
