摘要
目的比较分析两个不同厂家试剂检测EB病毒早期抗原IgA抗体(EA-IgA)、衣壳抗原IgA抗体(VCA-IgA),并做方法学评价。方法分别用两个不同厂家的ELISA试剂盒检测184例鼻咽癌(NPC)患者及184例正常人的血清EA-IgA和VCA-IgA。结果与欧蒙公司的试剂盒相比,IBL公司的试剂EA-IgA的阳性符合率为66.07%,阴性符合率为89.45%,总符合率为82.34%,Kappa值为0.57;VCA-IgA的阳性符合率为67.57%,阴性符合率为99.32%,总符合率为80.16%,Kappa值为0.62。结论两个厂家的试剂盒在分别验证EA-IgA,VCA-IgA时,具有高度一致性。国内IBL公司生产的试剂可替代进口试剂。
Objective To evaluate the two kits from two different manufacturers in detecting EA-IgA and VCA-IgA of Epstein-Barr virus,then provide the methodology evaluation.Methods The serum of EBVEA-IgA and VCA-IgA in 184 Nasopharyngeal patients and 184 healthy adults were detected use two kits.Results Compared with the kit of EUROIMMUN,for the kits of IBL to detect EA-IgA,the positive coincidence rate was 66.07%,negative coincidence rate was 89.45% and the total coincidence rate was 82.34%,with Kappa value of 0.57;for VCA-IgA,the positive coincidence rate was 67.57%,negative coincidence rate was 99.32% and the total coincidence rate was 80.16%,with Kappa value of 0.62.Conclusion The two kits have high consistence in screening of EA-IgA and VCA-IgA.The IBL kit produced by the domestic may be a substitute for imported reagents.
出处
《中国实用医药》
2011年第15期7-8,共2页
China Practical Medicine
关键词
EB病毒
鼻咽癌
EA-IgA
VCA-IGA
Epstein-Barr virus
Nasopharyngeal cancer
IgA antibodies against the early antigen of Epstein-Barr virus（EA-IgA）
IgA antibodies against the viral capsid antigen of Epstein-Barr virus（VCA-IgA）
