摘要
背景:胃癌对化疗药物原发或继发不敏感可致肿瘤多药耐药。目的:探讨胃癌组织中多药耐药相关蛋白P糖蛋白(P-gp)、谷胱甘肽S-转移酶(GST)-π、Bcl-xL的表达及其意义。方法:收集胃癌手术标本113例,每例标本选取肿瘤组织、距病灶5 cm以上的正常胃黏膜组织各1块。以免疫组化法检测多药耐药相关蛋白P-gp、GST-π、Bcl-xL的定位和表达,并与胃癌主要临床病理特征行相关性分析。结果:胃癌组织的P-gp、GST-π、Bcl-xL阳性表达率(48.7%对5.3%,P<0.05;62.8%对25.7%,P<0.05;83.2%对23.0%,P<0.05)和高表达率(18.6%对0%,P<0.05;23.9%对0%,P<0.05;71.7%对0%,P<0.05)均明显高于正常胃黏膜组织,胃癌组织P-gp、GST-π、Bcl-xL三者间表达呈正相关(P<0.05)。胃癌组织GST-π、Bcl-xL阳性表达与肿瘤组织分化程度呈正相关(P<0.05).胃癌组织P-gp、GST-π、Bcl-xL表达与患者的性别、年龄、浸润深度、淋巴结转移和临床分期均不相关(P>0.05)。结论:胃癌组织多药耐药相关蛋白P-gp、GST-π、Bcl-xL表达与胃癌的发生相关,且肿瘤组织GST-π、Bcl-xL表达与分化程度呈正相关,可作为判断胃癌患者预后的指标。
Background： The primary or secondary non-sensitivity of gastric cancer to chemotherapy induces muhidrug resistance. Aims： To investigate the expressions of muhidrug resistance-associated protein P-glycoprotein （P-gp）, glutathione S-transferase （GST）-π and Bcl-xL in tissues of gastric cancer and its significance. Methods： Cancerous specimens and normal gastric mueosal specimens （more than 5 cm away from the cancerous lesions） were obtained from 113 samples with gastric cancer. Immunohistochemical staining was used to detect the location and expression of P-gp, GST-π and Bcl-xL, and their relationships with major clinicopathological features of gastric cancer were analyzed. Results： The positive expression rates （48,7% vs. 5.3%, P〈0.05; 62.8% vs. 25.7%, P〈0.05; 83.2% vs. 23.0%, P〈0.05） and high expression rates （18.6% vs. 0%, P〈0.05; 23.9% vs. 0%, P〈0.05; 71.7% vs. 0%, P〈0.05） of P-gp, GST-π and Bcl-xL in gastric cancerous tissues were significantly higher than those in normal gastric mucosa. The expressions of P-gp, GST-π and Bcl-xL in cancerous tissues were positively correlated with each other （P〈0.05）. The expressions of GST-π and Bcl-xL in cancerous tissues were positively correlated with the differentiation of tumor （P〈0.05）, while no correlations were found between P-gp, GST-π and Bcl-xL expressions and patients＇ gender, age, depth of infiltration, lymph node metastasis or clinical stage （P〉0.05）. Conclusions： The expressions of multidrug resistance-associated protein P-gp, GST-π and Bcl-xL are related with gastric carcinogenesis. The expressions of GST-π and Bcl-xL in cancerous tissues are positively correlated with the differentiation of tumor, and could be used as prognostic factors in patients with gastric cancer.
出处
《胃肠病学》
2011年第5期293-297,共5页
Chinese Journal of Gastroenterology
