摘要
以啤酒厂不同处理工段废水、污泥为原料培养苏云金芽胞杆菌(Bt),并与城市污水做比较,研究啤酒废水、污泥的化学耗氧量(COD)、pH、发酵情况及其毒力.结果表明,以啤酒厂混合池原废水和酸化池废水为原料培养Bt,活芽胞数可达109 cfu.mL-1级,与城市污水相比(D595 nm=0.202),能产较高的晶体蛋白(D595 nm=0.209),发酵时间18.0-22.5 h.用啤酒废水培养Bt的发酵液毒力更强、发酵周期短、营养好,适合做为Bt发酵的工业化原料.
Sewage from different brewery working procedure was provided as media to cultivate Bacillus thuringiensis（Bt）.The chemical oxygen demand（COD）,pH,parameters of fermentation,and toxicity of sewage from urban sewage-digestive plant and brewery wastewater-digestive plant were investigated.It was showed that brewery wastewater/acidified wastewater＋sludge was suitable media,which could produce 109 cfu·mL-1 spores.Compared to urban wastewater＋sludge materials（D595 nm=0.202）,it had higher crystal compound（D595 nm=0.209） and shorter fermentation cycle with 18-22.5 h.It would be good material for Bt biopesticide production.
出处
《福建农林大学学报（自然科学版）》
CSCD
北大核心
2011年第3期296-301,共6页
Journal of Fujian Agriculture and Forestry University:Natural Science Edition
基金
科技部农业科技成果转化资金资助项目(2010GB2C400212)
教育部博士点基金资助项目(20093515110010
20093515120010)
卫生部科学研究基金资助项目(WKJ2008-2-44)
福建省高校服务海西建设重点项目(闽教高[2009]8号)
莆田市科技计划项目(2008N18)
关键词
苏云金芽胞杆菌
啤酒废水污泥
发酵
Bacillus thuringiensis
brewery wastewater/sludge
fermentation
