摘要
目的探讨血清Ⅰ型胃蛋白酶原(PGⅠ)和Ⅱ型胃蛋白酶原(PGⅡ)、胃蛋白酶原(PGⅠ/PGⅡ)比值、胃泌素-17(G-17)和幽门螺杆菌IgG抗体(IgG anti-HP)水平与萎缩性胃炎(AG)发生的关系及其诊断价值。方法分别采用胶乳免疫比浊法和酶联免疫吸附试验(ELISA)法检测48例AG患者及48名健康对照者血清PGⅠ、PGⅡ和G-17、IgG anti-HP浓度,采用受试者工作特征(ROC)曲线确立各指标筛查AG的最佳临临界值。结果与对照组相比,AG组血清PGI、PGⅠ/PGⅡ比值和G-17水平降低(P<0.01),而PGⅡ和IgG anti-HP水平升高(P<0.01);不同萎缩部位AG患者的血清PGⅠ、PGⅡ、PGⅠ/PGⅡ比值、G-17和IgG anti-HP水平差异有统计学意义(P<0.01)。根据ROC曲线,PGⅠ、PGⅠ/PGⅡ比值、G-17和IgG anti-HP的最佳临临界值分别为46.7 ng/L(敏感性82.4%,特异性80.0%,曲线下面积0.831±0.084)、4.60(敏感性94.4%,特异性85.7%,曲线下面积0.942±0.041)、7.45 pmol/L(敏感性50.0%,特异性72.2%,曲线下面积0.579±0.108)和78.9 IU(敏感性55.6%,特异性85.7%,曲线下面积0.746±0.089)。结论检测血清PGⅠ、PGⅠ/PGⅡ比值、G-17和IgG anti-HP水平可用于AG的筛查,PGⅠ/PGⅡ比值是诊断AG的最佳单一测量指标。
Objective To investigate the relationship between the serum pepsinogen （PG） Ⅰ , PG Ⅱ, PG Ⅰ/PG Ⅱ ratio, gastrin-17 （G-17）, IgG anti-Helicobacter pylori （IgG anti-HP） antibody and atrophic gastritis （AG） and their diagnostic values. Methods The levels of serum PG Ⅰ , PG Ⅱ , G-17 and IgG anti-HP were detected by emulsion immunoturbidimetry and enzyme-linked immunosorbent assay （ELISA） in 48 patients with AG and 48 healthy subjects as controls. The best cut-off value to screen AG was established according to the receiver operating characteristics （ROC） curve. Results The levels of serum PG Ⅰ, PG Ⅰ/PG Ⅱ ratio and G-17 in the AG group were significantly lower than those in the control group, while the levels of serum PG Ⅱ and IgG anti-HP were significantly higher than those in the control group （P 〈0.01 ）. Serum PGI, PG Ⅱ , PG Ⅰ/PG Ⅱ ratio, G-17 and IgG anti-HP levels in different atrophy regions had statistical significant difference （ P 〈 0.01 ）. According to the ROC curves, the best cut-off values of PG Ⅰ, PG Ⅰ/PG Ⅱ ratio, G-17, IgG anti-HP were 46.7ng/L （ sensitivity 82.4% ,specificity 80.0%, area under curve 0. 831 ± 0. 084）, 4.60 （ sensitivity 94.4%, specificity 85.7%, area under curve 0. 942 ± 0. 041 ）, 7.45 pmol/L （ sensitivity 50.0% ,specificity 72.2% ,area under curve 0.579 ±0. 108） and 78.9 IU （ sensitivity 55.6% ,specificity 85.7% ,area under curve 0.746 ± 0. 089）. Conclusions The detection of serum PG Ⅰ, PG Ⅰ/PG Ⅱ ratio, G-17 and IgG anti-HP levels can be useful to screen AG, and the PG Ⅰ/PG Ⅱ ratio may be the best single parameter for the diagnosis of AG.
出处
《检验医学》
CAS
北大核心
2011年第6期375-378,共4页
Laboratory Medicine
