摘要
笔者通过RT-qPCR分析了地黄中7个传统内参基因18S、EF-1a、ACT11、UBQ10、UBQ5、TUB5、GAPDH和4个新报道的内参基因PP2A、RPⅡ、HSP90、TIP41的mRNA表达差异情况,分别利用Ct值比较,Ge Norm、Norm Finer和BestKeeper软件分析它们在2个发育时期、8种不同组织器官中表达稳定性。结果表明:在地黄花器官中(花瓣、花托、雄蕊、雌蕊、子房),TIP41和UB Q10表达稳定;在地黄生殖生长期和营养生长期不同器官中(根、茎、叶),TIP41和UB Q5表达稳定。因此,以上2组基因分别适宜作为不同营养器官的内参基因。
A total of 11 reference genes were systematically compared in different tissues of Rehmannia glutinosa using RT-qPCR, including 7 traditional reference genes （ACT 11, 18S , TUB 5, GAPDH , EF- 1a , UBQ 10 andUBQ 5） and 4 novel candidates reference gene （HSP 90,PP 2A ,RPⅡ andTIP 41） in this study. The mRNA levels of these genes were analyzed among the organs of flowers, roots, stems and leaves representing the vegetative growth phase and the generative growth phase. The stabilities of the 11 candidate reference genes were ranked by the Ct value comparison and three softwares （GeNorm, NormFiner and BestKeeper）. The results showed that the expression ofTIP 41 andUBQ 10 was stable in different tissues of sepals, petals, stamens, pistils, and ovary from flower. For the case of the roots, stems and leaves sampled in vegetative growth phase and generative growth phase,UBQ 5 andTIP 41 were expressed stably. As a result, the 2 groups of candidate genes were selected as reference genes forRehmannia glutinosa.
出处
《中国农学通报》
CSCD
北大核心
2011年第17期76-82,共7页
Chinese Agricultural Science Bulletin
基金
国家科技重大专项课题"中药材种子种苗和种植(养殖)标准平台"(2009ZX09308-002)
