摘要
对液相色谱法测定山楂片中苯甲酸、山梨酸、糖精钠进行了方法优化,对色谱条件进行了调整,采用ODS—Cl8型(150mm×4.6mm,5μm)色谱柱,流动相甲醇～0.02mol/L乙酸铵(体积比5:95),流速为1.0mL/min,采用紫外检测器,检测波长为230nm。该方法测定结果的相对标准偏差小于2%(n=4),平均回收率大于90%,该法满足实验要求。
The method of determination of Benzoic Acid（sodium）,Sorbic Acid（potassium）,Saccharin Sodium in Haw Products by high performance liquid chromatography was optimized.the method of sample preparation was adjusted accordingly.The separation was achieved by using a C18 column（150mm×4.6mm）with mobile phase 0.02mol/L NH4Ac/methanol（95/5）,lastly the determination was conducted at 230 nm through a UV detector.The average recovery of the method in real samples was more than 95.25%,and the RSD was less than 2%,the method is suitable for the need of experiment.
出处
《江西食品工业》
2011年第2期41-42,共2页
Jiangxi Food Industry
关键词
高效液相色谱法
山楂片
苯甲酸、山梨酸、糖精钠
HPLC
Haw Products
Benzoic Acid（sodium）、 Sorbic Acid（potassium）、 Saccharin Sodium
