摘要
目的:建立同时测定杜仲雄花中京尼平苷酸、绿原酸、栀子苷3种成分含量的方法。方法:采用双波长高效液相色谱法。色谱柱为Agilent Zorbax Extend C18(250mm×4.6mm,5μm),流动相为甲醇-水-醋酸(15∶85∶1),检测波长为238nm(测定京尼平苷酸和栀子苷)和327nm(测定绿原酸),流速为1mL·min-1,柱温为30℃。结果:京尼平苷酸、绿原酸、栀子苷的进样量线性范围分别为0.0892～5.3520μg(r=0.9995)、0.1052～6.3120μg(r=0.9995)、0.1024～6.1440μg(r=0.9995);三者平均加样回收率分别为99.24%、98.86、101.44%,RSD分别为2.17%、1.95%、2.08%(n=6)。结论:双波长或多波长法能方便、快速地对中药中多种成分同时测定,能满足中药多成分质量控制的要求。
OBJECTIVE：To develop the method for the content determination of geniposidic acid,chlorogenic acid and geniposide in male flowers of Eucommia ulmoides.METHODS：Dual-wavelength HPLC was adopted.The analytical column was Agilent Zorbax Extend C18（250 mm×4.6 mm,5 μm） column with mobile phase consisted of methanol-water-acetic acid（15 ：85 ：1） at flow rate of 1 mL·min-1.The detection wavelength was set at 238 nm for geniposidic acid and geniposide,and at 327 nm for chlorogenic acid.The column temperature was 30 ℃.RESULTS：The linear ranges of geniposidic acid,chlorogenic acid and geniposide were 0.089 2～5.352 0 μg（r=0.999 5）,0.105 2～6.312 0 μg（r=0.999 5）,0.102 4～6.144 0 μg（r=0.999 5）.Average recoveries of them were 99.24%,98.86%,101.44% and RSDs were 2.17%,1.95%,2.08%,respectively（n=6）.CONCLUSION：Dual or more wavelength HPLC method can be used for simultaneous determination of ingredients of traditional Chinese medicine,and can satisfy the requirements of quality control.
出处
《中国药房》
CAS
CSCD
北大核心
2011年第27期2565-2567,共3页
China Pharmacy
关键词
杜仲
雄花
京尼平苷酸
绿原酸
栀子苷
双波长高效液相色谱法
含量测定
Eucommia ulmoides
Male flowers
Geniposidic acid
Chlorogenic acid
Geniposide
Dual-wavelength HPLC
Content determination
