摘要
目的 分析恶性骨肿瘤化疗药物敏感性和多药耐药性 ,为制定敏感的个体化化疗方案提供依据。 方法 取恶性骨肿瘤新鲜标本 3 2例 (骨肉瘤 2 0例 ,其它恶性肿瘤 12例 ) ,运用流式细胞术检测化疗药物作用后的瘤细胞凋亡情况及其P170蛋白表达 ,分析其化疗敏感性和耐药性。结果 不同化疗药物作用后的骨肉瘤细胞凋亡百分比分别为 :甲氨喋呤 ( 3 0 5 0± 10 2 2 ) % ,阿霉素( 2 6 2 8± 9 3 5 ) % ,丝裂霉素 ( 2 3 11± 7 3 8) % ,足叶乙甙 ( 18 17± 6 14 ) % ,长春新碱 ( 4 4 4± 2 5 5 ) % ,环磷酰胺 ( 1 2 2± 0 5 9) %。P170蛋白表达较高者化疗敏感性均较低。 结论 流式细胞术分析化疗药物作用后的恶性骨肿瘤细胞凋亡及其P170蛋白表达 ,对预测肿瘤的化疗敏感性和耐药性有一定意义。
Objective To study chemosensitivity and multidrug resistance of malignant bone tumors and to make an individual protocol of chemotherapy with sensitive antitumor agents. Methods Apoptosis induced by MTX,ADM,MMC,VP 16,VCR and CTX,and chemosensitivity in 32 fresh specimens of malignant bone tumors were onalyzed by flow cytometry(FCM) and quantitative immunofluorescence. The expression of P glycoprotein(P170) in the speciments and the relation between P170, multidrug resistance, and chemosensitivity were detected. Results The rate of apoptosis was significantly different in malignant bone tumor cells treated with different durgs: MTX(30 50±10 22)%,ADM(26 28±9 35)%,MMC(23 11±7 38)%,VP 16(18 17±6 14)%,VCR(4 44±2 55)%,and CTX(1 22±0 59)%.The individual variation of cell apoptosis was prominent in the specimens of same pathological type treated with same agent.There was lower chemosensitivity in the malignant bone tumors with a high expression of P170. Conclusions Chemosensitivity testing by flow cytometry is a simple,quick and sensitive assay.Quantitative analysis of expression of P170 could be used to predict multidrug resistance and its chemosensitivity of malignant bone tumors.
出处
《中华外科杂志》
CAS
CSCD
北大核心
1999年第12期737-739,共3页
Chinese Journal of Surgery
基金
四川省卫生厅科研基金
