摘要
目的观察脊髓损伤后BMPRIa型受体的表达。方法运用免疫组织化学方法,首先检测3种BMP受体(BMPR)Ia、Ib、II型在正常成体大鼠脊髓中的表达分布。运用大鼠钝性脊髓损伤模型,以150kdyn的撞击力直接撞击脊髓,观察动物撞击后1、3、7、14、30、60d后脊髓中BMPRIa的表达改变。结果在正常成年大鼠脊髓中,BMPRIa、II型受体主要在少突胶质细胞、灰质神经元中表达,在部分星形胶质细胞和大多数小胶质细胞中表达。灰质神经元中未检测到Ib型受体的表达或表达很低。脊髓损伤后,BMPRIa在星形胶质细胞中表达激剧增加,高表达可持续至损伤后1个月;脊髓损伤诱导脊髓小胶质细胞活化,活化的小胶质细胞中表达BMPRIa明显增加。结论大鼠脊髓损伤后,诱导BMPRIa受体在星形胶质细胞、小胶质细胞激剧增加,BMPRIa高表达提示BMP信号在胶质细胞的重要病理生理作用,这一发现为进一步研究BMP信号的功能作用提供基础。
Objective To observe the expression pattern of bone morphogenetic protein receptor IA（BMPR IA） in rats after contusive spinal cord injury.Methods The expressions of BMPR IA,IB,and II were detected by immunochemistry in the spinal cord of normal adult rats,and the expression of BMPR IA was detected in the infinite horizons impactor model at 1,3,7,14,30,and 60 days after spinal cord injury.Results In the spinal cord of normal adult rats,BMPR IA and II were expressed predominantly in the oligodentrocytes and neurons in the grey matter,and also in some astrocytes and numerous microglia cells.Only a low level of BMPR IB expression was detected in the neurons of the grey matter.After spinal cord injury,the expression of BMP IA markedly increased with sustained strong expression in the astrocytes till one month after the injury;its expression was also increased obviously in the microglia cells activated by the injury.Conclusion The expression of BMPR IA increases significantly in the astrocytes and activated microglia cells in rats after contusive spinal cord injury,suggesting the involvement of BMP signaling pathway in the physiological and pathological role of glia cells.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2011年第7期1124-1130,共7页
Journal of Southern Medical University
基金
国家自然科学基金(81070994)
教育部留学回国人员科研启动基金(教外司留(2009)8号文)~~
