摘要
目的测定中药戒毒康酊剂中氢溴酸东莨菪碱及总生物碱的含量。方法采用Diamonsil C18柱(250 mm×4.6 mm,5μm)分离测定氢溴酸东莨菪碱,流动相:以甲醇-0.02 mol.L-1醋酸钠溶液(含0.02%三乙胺和0.3%四氢呋喃,用冰醋酸调pH到6.2)梯度洗脱;流速1 mL.min-1;检测波长:206 nm。采用酸性染料比色法测定戒毒康中的总生物碱,0.2%溴百里香酚兰溶液显色,420 nm处测得吸光度。结果氢溴酸东莨菪碱在1.0～5.0μg内呈良好的线性关系(r=0.999 6);平均回收率为98.8%,RSD为2.29%(n=6)。总生物碱在0.04～0.2 mg内呈良好的线性关系(r=0.999 8);平均回收率为97.6%,RSD为2.06%(n=6)。结论本方法简便、快速、准确、专属性强,可作为戒毒康酊剂的检验方法。
OBJECTIVE To establish an HPLC method for determination of contents of scopolamine hydrobromide and total alkaloid in Jiedukang tincture. METHODS A Diamonsil C18 column（250 mm×4.6 mm, 5μm） was used to isolate and determine scopolamine hydrobromide. The mobile phase was methanol-0.02 mol.L^-1 NaAc solution （with 0.02% triethylamine and 0.3% tetrahydrofuran, adjusted to pH 6.2 by HAc）. The flow rate was 1 mL.minH. The detection wavelength was set at 206 nm. Total alkaloids in Jiedukang tincture were determined with acid dye colorimetry. 0.2% Bromothymol blue was used as the dye reagent and the wavelength was set at 420 nm. RESULTS The linear range of scopolamine hydrobromide was 1.0-5.0 μg with a good correlation （r=0.999 6）. The average recoveries was 98.8% with RSD of 2.29%（n=6）. The total alkaloids showed good linearity within the range of 0.04-0.2 mg（r=0.999 8）, the average recoveries was 97.6% with RSD of 2.06%（n=6）.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2011年第7期673-676,共4页
Chinese Journal of Modern Applied Pharmacy
关键词
氢溴酸东莨菪碱
高效液相色谱法
总生物碱
The method established is sensitive, accurate and can be used for determination of scopolamine hydrobromide
