摘要
目的:探讨骨保护素(Osteoprotegerin,OPG)、骨保护素配体(Osteoprotegerin ligand,OPGL)和环氧合酶-2(Cyclooxygenase-2,COX-2)在下颌骨缺损修复中的作用机制。方法:健康雄性新西兰大白兔48只,随机分为对照组(24只)和实验组(24只),建立下颌骨缺损模型,分别于建模后3、14、28、56d处死相同例数动物(n=6),然后收集下颌骨缺损处的骨组织块。采用免疫组织化学、RT-PCR技术检测OPG、OPGL、COX-2蛋白及mRNA表达变化。结果:兔下颌骨缺损修复过程中,OPG、OPGL、COX-2蛋白和mRNA在不同时间点均有不同程度高表达(P<0.05),但各指标峰值出现的时间点不同(P<0.01)。OPG,OPGL,COX-2峰值分别出现在建模后3、28、14d。结论:兔下颌骨缺损修复中OPG、OPGL、COX-2蛋白及mRNA表达水平不同程度高表达,提示其可能参与兔下颌骨缺损修复的过程。
Objective To explore the repair mechanism of osteoprotegerin （OPG）, osteoprotegerin ligand （OPGL） and cyclooxygenase-2（COX-2） in mandibular defect. Methods 48 healthy male New Zealand rabbits were randomly divided into the control group （24） and the experimental group （24）. On 3 d, 14 d, 28 d, 56 d after the mandibular defect modele was established, 6 rabbits were killed separately. At the same time, the bone in the zone of mandibular defect was collected. The expression of protein and mRNA including OPG, OPGL and COX-2 were tested by means of immunohistoehemical technique and RT-PCR. Results The protein and mRNA of OPG, OPGL and COX-2 were highly expressed to varying degrees at different time points （P 〈 0.05）. However, the maximum peak of three indexes presented at different time points separately （P 〈 0.01 ）. OPG, OPGL, COX-2 presented their each maximum peak on 3 d, 28 d, 14 d respectively after establishing model. Conclusion The protein and mRNA of OPG, OPGL and COX-2 were highly expressed to varying degrees at different time points, which can be concluded that they may oarticioate in the repairing process of the mandibular defect.
出处
《实用医学杂志》
CAS
北大核心
2011年第15期2712-2714,共3页
The Journal of Practical Medicine
