摘要
应用~3H-TdR光镜和电镜放射自显影观察表明,MGc80-3 细胞标记指数达31.51％,标记银粒集中于分裂间期的S期细胞核中,细胞核呈重标记状态,绝大多数标记银粒分布于细胞核常染色质区域,少数见于核孔附近的常染色质或核孔附近的细胞质中,核仁未见标记,显示细胞内 DNA合成十分活跃。但经 dBcAMP诱导后,标记指数则降至 4.35％,细胞核呈弱标记状态,电镜放射自显影则未在细胞核内见到标记银粒,表明其DNA合成受到明显抑制。这种变化是由于dBcAMP诱导胃癌细胞内cAMP水平提高而实现的,认为DNA合成抑制是导致MGc80-3细胞走向分化的一个重要原因,对于癌变细胞恶性表型逆转具有重要作用。
The autoradiographic observation with light and electron microscopy indicated that, in MGc80-3 cells, many heavy labeling nuclei could be seen, the labeling exponent amounted to 31.51 %. The labeling silver granules were concentrated in the nuclei of divided interval cells, most of them were distritut ed within the euchromatin zone, a few silver granules were found at the euchrornatin or cytoplasm nearby nuclear pores, but could not be seen at nucleoli. tt showed that their DNA synthesis was very active But in the cells after dBcAMP treatment, only a few weak labeling nuclei could be found, their labeling exponent decreased to 4.35%. The labeling silver granules could not be found in their nuclei under the electron microscope. It showed that the DNA synthesis had been inhibited remarkably. This change was realized by the enhancing of cAMP level in gastric carcfnoma cells induced by dBcAMP. The inhibition of DNA synthesis is one of the important reasons of MGc80-3 cell differentiation .It has an important action on the malignant phenotypic reversion of carcinoma cells.
出处
《厦门大学学报（自然科学版）》
CAS
CSCD
北大核心
1990年第5期565-569,共5页
Journal of Xiamen University：Natural Science
关键词
DBCAMP
人体
胃腺癌细胞
体外培养
Dibutyryl cAMP. Human gastric adenocarcinoma cell, DNA synthesis
