摘要
目的:寻找预测体外受精-胚胎移植(IVF-ET)中受精失败的可能因素。方法:回顾性分析IVF-ET中完全受精失败患者(研究组,n=32)不孕年限、发病史、促排天数、hCG注射日的性激素水平[LH、E2和孕酮(P)]、精子表面抗体IgG混合凝集反应(MRA)、抗精子抗体、精子畸形率等指标,并匹配同一日取卵,放在同一培养箱受精率≥50%的IVF-ET患者作为对照组(n=56)。结果:研究组在不孕年限、超促排卵天数、原发比例、hCG注射日P水平>3.12 nmol/L的比例及严重畸形精子(畸形率>95%)比例显著高于对照组,分别为6.4±3.1年、12.6±2.2 d、56%、43%、43%比4.6±2.9年、11.6±1.3 d、33%、23%、23%(P<0.05或P<0.01)。结论:原发性不孕患者,不孕年限及超促排卵天数较长(>6.4年和>12.6 d)同时合并有hCG注射日P水平升高(>3.12 nmol/L)或畸精子率>95%,应将该患者纳入受精失败的高危人群。
Objective： To find the possible factors predicting fertilization failure of in vitro fertilization-embryo transfer（IVF-ET）.Methods： The IVF-ET patients with complete fertilization failure（experimental group,n=32） were analyzed retrospectively.The patients whose oocytes retrieved at the same day and cultured on the same incubators with ≥ 50% fertilization rates were matched as the control（n=56）.Results： The infertile period,superovulation days,the rates of primary case,progesterone（P） level 〉3.12 nmol/L rates and rates of severe abnormal sperm in experimental group were significantly higher than those in the control（6.4 ± 3.1 years,12.6±2.2 d,56%,43%,43% vs 4.6 ± 2.9 years,11.6±1.3 d,33%,23%,23%,respectively,P0.05）.Conclusion： We should pay attention to these patients with primary infertility,infertility duration and superovulation days longer（〉 6.4 years and〉 12.6 d） and having increased rates of the value of hCG injection day at P level〉 3.12 nmol/L,rate abnormal sperm〉95% at the same time.They should be included in such patients at high risk of fertilization failure.
出处
《生殖与避孕》
CAS
CSCD
北大核心
2011年第7期455-458,480,共5页
Reproduction and Contraception
基金
广东省卫生厅基金资助项目
项目号:B2009110
关键词
体外受精-胚胎移植(IVF-ET)
完全受精失败
精子畸形率
in vitro fertilization-embryo transfer（IVF-ET）
complete fertilization failure
proportion of sperm abnormality
