摘要
We investigated the compositional and structural differences in sequences derived from different fractions of wheat genomic DNA obtained using methylation filtration and Cot fractionation. Comparative analysis of these sequences revealed large compositional and structural variations in terms of GC content, different structural elements including repeat sequences （e.g., transposable elements and simple sequence repeats）, protein coding genes, and non-coding RNA genes. A correlation between methylation status [determined on the basis of selective inclusion/ exclusion in methylation-filtered （MF） library] of different repeat elements and expression level was observed. The expression levels were determined by comparing MF sequences with expressed sequence tags （ESTs） available in the public domain. Only a limited overlap among ME high Cot （HC）, and ESTs was observed, suggesting that these sequences may largely either represent the low-copy non-transcribed sequences or include genes with low expression levels. Thus, these results indicated a need to study MF and HC sequences along with ESTs to fully appreciate complexity of wheat gene space.
We investigated the compositional and structural differences in sequences derived from different fractions of wheat genomic DNA obtained using methylation filtration and Cot fractionation. Comparative analysis of these sequences revealed large compositional and structural variations in terms of GC content, different structural elements including repeat sequences （e.g., transposable elements and simple sequence repeats）, protein coding genes, and non-coding RNA genes. A correlation between methylation status [determined on the basis of selective inclusion/ exclusion in methylation-filtered （MF） library] of different repeat elements and expression level was observed. The expression levels were determined by comparing MF sequences with expressed sequence tags （ESTs） available in the public domain. Only a limited overlap among ME high Cot （HC）, and ESTs was observed, suggesting that these sequences may largely either represent the low-copy non-transcribed sequences or include genes with low expression levels. Thus, these results indicated a need to study MF and HC sequences along with ESTs to fully appreciate complexity of wheat gene space.
基金
supported by the Government of India's Departments of Biotechnology,and Science and Technology, Indian National Science Academy,and National Institute of Health grant(No.1 R01 GM080749-01A1)
