摘要
目的探讨医院重症监护病房(ICU)肺炎克雷伯菌(KPN)医院感染现状及耐药性,为临床医师合理使用抗菌药物提供科学依据。方法了解229株KPN医院感染的标本来源分布;严格按照《全国临床检验操作规程》进行细菌培养;采用法国生物梅里埃公司VITEK-32全自动微生物分析仪鉴定菌株;采用K-B法进行药敏试验,结果按CLSI折点进行评价。结果 ICU内KPN除对碳青霉烯类抗菌药物亚胺培南敏感率为100.0%以外,对其他常用抗菌药物均产生了不同程度的耐药性。结论医院应狠抓细菌耐药性的监测与控制工作,预防多药耐药菌在医院内传播与蔓延。
OBJECTIVE To investigate the antimicrobial resistance in Klebsiella pneumoniae（KPN） in intensive care unit and provide scientific evidence for clinically reasonable use of antibiotics.METHODS Retrospective review was performed to analyze the specimen source and the clinical distribution for 229 isolates of KPN.Referring to National Guide to Clinical Laboratory Procedures,bacteria cultivation was carried out.The VITEK-32 identification system（France bioMérieux） was used to identify the strains.The susceptibility testing was performed by K-B methods.And susceptibility testing results were assessed according to CLSI breakpoints.RESULTS The results of susceptibility testing indicated that KPN was resistant to the common antibiotics in varying degree except for 100.0% sensitivity to carbapenem antibiotics such as imipenem.CONCLUSION The hospitals should strengthen the monitoring and controlling of antibiotical resistance to prevent the occurrence and prevalence of multidrug-resistant strains causing nosocomial infection.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2011年第16期3498-3499,共2页
Chinese Journal of Nosocomiology
关键词
医院感染
肺炎克雷伯菌
重症监护病房
耐药性
Nosocomial infection
Klebsiella pneumoniae
Intensive care unit
Antimicrobial resistance
