摘要
采用电化学发光免疫分析法(ECLIA)同时测定甲状腺球蛋白(Tg)、自身甲状腺球蛋白抗体(TgAb)值,并用回收实验研究TgAb对Tg测定值的影响。甲状腺疾病患者血样84例,测定Tg、TgAb。回收实验分为三组。第一组分别加入50、100和200 ng/ml的标准Tg;第二组TgAb≥4 000 IU/ml的血样对倍稀释5次,分别测定Tg、TgAb值,并加入100 ng/ml标准Tg;第三组TgAb浓度梯度组,分别加入100 ng/ml标准Tg。计算回收率(R)。结果显示血清对倍稀释后,TgAb实测值逐渐下降;Tg实测值、Tg计算值逐渐上升,两者的差值随稀释倍数的增加而增大。当R<80%时,有22人(91.7%)TgAb>115 IU/ml。ECLIA测定Tg时,TgAb的干扰导致Tg测定值低于真实值,并呈浓度依赖性。TgAb的存在是导致回收率降低的主要原因。
Serum thyroglobulin（Tg） is primarily used as a tumor marker to detect the recurrent or persistent disease in patients with differentiated thyroid carcinomas.Unfortunately,the serum Tg measurement is technically challenging and the thyroglobulin autoantibody（TgAb） interference remain the most serious problem limiting the clinical value of serum Tg.The direction and magnitude of the interference are related to the method and the concentration and affinity of the TgAb in the specimen.The objective of this study was to evaluate TgAb＇s interference with assay of Tg by electrochemiluminescent assay（ECLIA）.The Tg and TgAb of 84 sera were measured by the ECLIA.Recovery tests were carried out in 3 groups.3 different Tg calibrators,50,100,200 ng/ml,respectively,were added into the sera of the first group.The sera of second group were doubly diluted 5 times,and meawhile the Tg and TgAb were measured after each dilution,and then 100ng/ml Tg calibrator was added into the sera.The sera of third group were divided into different subgroups according to TgAb concentration and then 100ng/ml Tg calibrators were added.Recovery rate（%） was calculated.Tg value decreased when TgAb concentration increased by ECLIA,TgAb value became lower when sera were diluted,Tg value increased.The added Tg calibrator had not significant influence on Tg value and Recovery rate.Recovery rate was lower when TgAb concentration increased.When sera were diluted,the recovery rate was increased.Tg value were underestimated by TgAb interference.After sera were diluted,Tg value became increasing by ECLIA.The added Tg calibrator had not significantly influence on Tg value and Recovery rate.When sera were diluted,the recovery rate was increased.TgAb concentration had significant influence on Recovery rate.Rrecovery test can not efficiently rectify Tg value when TgAb was positive.
出处
《生物医学工程学杂志》
EI
CAS
CSCD
北大核心
2011年第4期780-783,共4页
Journal of Biomedical Engineering
基金
国家自然科学基金资助项目(30870724)
关键词
甲状腺球蛋白
自身甲状腺球蛋白抗体
分化型甲状腺癌
电化学发光免疫分析法
回收实验
Thyroglobulin（Tg）
Thyroglobulin autoantibody（TgAb）
Differentiated thyroid carcinomas（DTC）
Electrochemiluminescent assay（ECLIA）
Recovery test
