摘要
目的:研究经颅电刺激加康复训练能否促进大鼠局部脑缺血再灌后的功能恢复并从神经可塑性角度探讨其机制。方法:线栓法制备SD大鼠短暂大脑中动脉缺血再灌模型(MCAO),术后随机分为电刺激加康复组(A)、康复组(B)、对照组(C)。A组给予经颅电刺激、滚笼和平衡木训练。B组仅给予滚笼和平衡木训练。分别于术后3、7、14和28天用走横木试验(BWT)进行运动功能评分,以及用免疫组化法检测微管相关蛋白-2(MAP-2)在梗死灶周围的免疫活性强度。结果:A组BWT评分在第7、14、28天优于B组和C组,差异有显著性意义(P<0.05);A组皮质缺血周边区MAP-2表达水平在第14、28天高于B组和C组,差异有显著性意义(P<0.05)。结论:经颅电刺激加康复训练能促进大鼠MCAO后肢体功能恢复,其机制可能与上调缺血灶周边MAP-2的表达有关,上述作用优于单独康复训练。
Objective： To assess the influence of transcranial electric stimulation （TES） combined with rehabilitative training on brain plasticity and behavioral functional performance in rats following a focal ischemia-reperfusion insult.Method： After an acute focal ischemic insuh developed by transient occlusion of right middle cerebral artery. （MCAO）, the TES combined with rehabilitation group（A） were given TES, balancing, rotating and walking exercises everyday; the rehabilitation group（B） were given only balancing, rotating and walking exercises everyday; the con-trol group（C） was without any treatment. Microtubule-associated protein-2（MAP-2） were examined by immunohisto-chemical techniques, and density of reaction product and narrow-beam-walking tasks（BWT） were measured at the 3rd ,7th,14th and 28th d after infarction respectively.Result：A group provided marked improvement in BWT at the 7th,14th and 28th d compared with B group and C group（P〈0.05）. The expression of MAP-2 demonstrated statistically significant increases at the 14th and 28th d in the peri-infarct regiones in A group compared with other two groups（P〈0.05）.Conclusion：These results support the efficacy of combining rehabilitative training with TES on improving function-al outcome and neuronal plasticity following ischemic cerebral damage. The function enhancement may he partly due to the up-regulation of expression of MAP-2 in peri-infarct regions. Meanwhile, this efficacy is superior to rehabilitative training only.
出处
《中国康复医学杂志》
CAS
CSCD
北大核心
2011年第8期750-753,共4页
Chinese Journal of Rehabilitation Medicine
关键词
脑梗死
经颅电刺激
康复训练
微管相关蛋白-2
大鼠
cerebral ischemic infarction
transcranial electric stimulation
rehabilitative training
microtubule-associat-ed protein-2
rats
