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应用EP7-A2方案评价循环酶法同型半胱氨酸试剂对其他生化检测项目的干扰 被引量:2

Application EP7-A2 Scheme Evaluation Circulation Enzymatic Homocysteine Reagent for Interference with Other Items on Board Same Analyzer
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摘要 目的:研究循环酶法同型半胱氨酸检测试剂对尿酸等项目检测的干扰。方法:用CLSIEP7-A2方案,评估循环酶法同型半胱氨酸试剂在生化分析仪中,因携带交叉污染对常用项目检测的干扰。结果:检测结果显示,在模拟污染率0.1%-0.5%范围内,ALT、AST从0.1%污染率开始,结果均超出(X-1.96s)范围;GSP从0.2%污染率开始,结果均超出(X+1.96s)范围;UA从0.3%污染率开始,结果均超过(X-1.96s)范围;Mg从0.3%污染率开始,结果均超出(X+1.96s)范围;其他15项目检测结果均在(X±1.96s)范围内。结论:生化分析仪携带污染率在0.5%的允许范围内,循环酶法同型半胱氨酸检测试剂可能对同一检测系统中,糖化血清蛋白(GSP)等5项检测造成干扰,而对总胆红素等其他15项检测不造成干扰。 Objective:To study uric acid et.detecting interferenced of the circulation enzymatic homocysteine reagent.Methods:Using CLSI EP7-A2 scheme,evaluate circular enzymatic homocysteine reagent in biochemistry analyzer,because carry cross contamination of interference to commonly used analysis items detection.Results:The simulation tests showed that the rate of pollution within 0.1%~0.5%,from 0.1% rate of pollution,the ALT and AST test results exceed(X-1.96s) limit;from 0.2% rate of pollution,the GSP test results exceed(X+1.96s) limit;from 0.3% rate of pollution,the uric acid test results exceed(X-1.96s) limit;from 0.3% rate of pollution,the Mg test results exceed(X+1.96s) limit.Other 15 projects are testing results within(X±1.96s).Conclusion:Biochemical analyzer to carry the rate of pollution in the range of allowable 0.5%,circulation enzymatic homocysteine reagent in the same detection system,saccharification serum protein(GSP) and five test cause interference,but to total bilirubin and other 15 test don't cause interference.
出处 《实用中西医结合临床》 2011年第4期67-68,共2页 Practical Clinical Journal of Integrated Traditional Chinese and Western Medicine
关键词 同型半胱氨酸 糖化血清蛋白 生化分析仪 携带交叉污染 Homocysteine(HCY) Saccharification serum protein(GSP) Biochemical analyzer Carry cross contamination
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