摘要
目的探讨阿魏酸对人角质形成细胞(human keratinocytes,HKC)的保护作用及作用机制。方法用氯化钴(CoCl2)处理HKC,建立氧化应激所致HKC炎症损伤细胞模型,检测阿魏酸预处理HKC后的相关指标,以3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide,MTT]法检测细胞生存率,用酶联免疫(enzyme linked immunosorbent assay,ELISA)法检测细胞培养液中肿瘤坏死因子α(tumor necrosis factorα,TNF-α)和白细胞介素8(interleukin-8I,L-8)的水平。结果正常对照组HKC细胞生存率100.00%,TNF-α和IL-8含量分别为11.37和98.30 pg.mL-1。0.03和0.3 mg.mL-1阿魏酸处理组中,HKC存活率(分别为96.15%和67.92%)亦显著高于模型组(46.20%,P<0.01,P<0.05),该作用呈剂量依赖性;TNF-α含量(分别为12.95和17.46 pg.mL-1)显著低于模型组(29.54 pg.mL-1,P<0.01,P<0.05);IL-8含量(分别为102.76和128.96 pg.mL-1)亦显著低于模型组(162.33 pg.mL-1,P<0.01,P<0.05)。0.003 mg.mL-1阿魏酸对HKC存活率和分泌TNF-α和IL-8无明显影响。结论阿魏酸可能通过抑制TNF-α和IL-8的释放发挥其对HKC的保护作用。
Objective To investigate the protective effects and mechanism of ferulic acid on human keratinocytes （HKC）. Methods The cell model of oxidative stress was established in HKC treated with CoC12. The cell viability post cultured with ferulic acid was detected by MTT method, the secretion of cytokines TNF-α and IL-8 in the supernatant was measured by ELISA. Results The surviving rate of HKC cell with normal group was 100. 00% and the contents of TNF-α and IL-8 was 11.37, 98.30 pg · mL^-1 respectively. The surviving rate of HKC cell respectively was 96. 15% ,67.92% in group treated with 0. 03 and 0.3 mg · mL^-1 Ferulic acid,which were significantly higher than those in the model group （46.20%, P〈0.01, P〈0.05 ）, and the effect was in a dose-dependent manner. The contents of TNF-α（respectively were 12.95 , 17.46 pg · mL^-1 ）were significantly lower than those in the mode] group （29. 54 pg · mL^-1, P〈0. 01, P〈0. 05 ）, The contents of IL-8 （respectively were 102. 76, 128.96 pg · mL^-1 ）were significantly lower than those in the model group（ 162.33 pg · mL^-1 ,P〈0.01 ,P〈0.05 ）. However, There were no significant effects on the surviving rate of HKC cell and the contents of TNF-α and IL-8 in group treated with 0.003 mg · mL^-1 Ferulic acid. Conclusion Ferulic acid may prevent HKC against oxidative injury by inhibiting secretion of TNF-α and IL-8.
出处
《医药导报》
CAS
2011年第8期982-984,共3页
Herald of Medicine
基金
湖北医药学院附属太和医院基金项目(基金编号:2009D30)
