摘要
目的探讨β-catenin基因在食管鳞状细胞癌(Esophageal squamous cell carcinoma,ESCC)中的表达及其意义。方法免疫组化法检测65例ESCC及20例因ESCC手术切除的远切端正常食管黏膜组织中β-catenin的表达及定位,分析其与临床病理学参数的关系;RT-PCR检测31例新鲜ESCC及相应的远切断正常食管黏膜组织中β-catenin mRNA的表达。结果免疫组化结果显示:在20例因ESCC手术切除的远切端正常食管黏膜组织中,β-catenin主要位于胞膜;在65例ESCC组织中,β-catenin的胞质积累阳性率为63.1%,胞核积累阳性率为30.8%;β-catenin的胞质/核积累与淋巴结转移相关(P=0.005),但与患者的年龄、性别、肿瘤的分化程度及浸润深度等无关。RT-PCR结果显示:在31例ESCC及相应的远切端正常食管黏膜组织中,均检测到β-catenin mRNA的表达,与相应的远切端正常食管黏膜组织比较,癌组织中β-catenin基因mRNA的表达水平明显升高(P=0.037),其中表达升高的21例,占67.7%,表达无明显差异的8例,占25.8%,表达降低的2例,占6.5%。结论在ESCC中存在β-catenin mRNA表达水平的升高以及β-catenin蛋白的异常胞质/胞核积累,并与淋巴结转移有关。
Objective To explore the expression and significance of β-catenin in esophageal squamous cell carcinoma（ESCC）.Methods Immunohistochemiscal staining was used to examine the expression of β-catenin protein in 65 cases of formalin-fixed,paraffin-embeded ESCC tissue（n=65） and their corresponding nonneoplastic epithelial tissues（n=20）.RT-PCR was used to determine the expression of β-catenin in 31 cases of fresh ESCC tissue（n=31） and their corresponding nonneoplastic epithelial tissues（n=31）.Results In the 20 samples of normal esophageal mucosa,immunohistochemistry showed that β-catenin was mainly located in the membrane,while 41 in 65（63.1%） tumor samples had cytoplasmic accumulation of β-catenin and 20（30.8%） had nuclear staining.The cytoplasmic/nuclear β-catenin was significantly associated with lymph node metastasis（P=0.005）,but not with the patient＇s age,gender,tumor grade or differentiation grade.In 31 fresh ESCC tissues studied,all tumor samples and paired normal samples expressed β-catenin mRNA.Twenty-one of 31（66.7%） tumors expressed β-catenin mRNA at a higher level than their corresponding nonneoplastic tissues,8 of 31（25.8%） were almost the same,and 2 of 31（6.5%） showed lower expression.Altogether,tumor tissues expressed a significantly higher level of β-catenin mRNA than the corresponding nonneoplastic tissues（P=0.037）.Conclusion ESCC shows an increased expression of β-catenin mRNA,and the abnormal cytoplasmic/nuclear accumulation of β-catenin is significantly associated with lymph node metastasis.
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
2011年第2期124-129,共6页
Chinese Journal of Histochemistry and Cytochemistry
基金
山西省自然科学基金资助(2010011047-5)
