摘要
目的:研究金疮小草醇提物(ADT)对正常小鼠凝血时间及微循环影响及抗急性肝损伤的作用。方法:正常小鼠50只,随机分成5组,即空白对照组、阿司匹林组、ADT高、中、低剂量组i,g给药1,次/d,连续7 d,末次给药后1 h,采用毛细管法及玻片法测定其对凝血时间的影响;测定肠系膜毛细血管网交点数、管径、血液流速以观察其对微循环的影响;正常小鼠60只,随机分成6组,即空白对照组、模型对照组、联苯双酯组、ADT高、中、低剂量组i,g给药,1天1次,连续7 d,末次给药后禁食不禁水8 h,除空白组外ip CCl4或D-GalN,12～16 h后取血分离血清,测定小鼠血清中ALT,AST水平以评价其对急性肝损伤的影响。结果:与空白对照组比较,ADT能明显延长小鼠血液的凝血时间(P<0.05),玻片法测得的凝血时间延长40%以上,毛细管法测得的凝血时间延长20%以上;显著增加肠系膜的毛细血管网交点数和加快微循环血液速度(P<0.05),网交点数增加了约30%,血液流速加快了约10%;与模型对照组比较,ADT能明显降低CCl4和D-GalN引起急性肝损伤动物的血清ALT,AST水平(P<0.05)。结论:金疮小草醇提物活血化瘀作用可能是抗急性肝损伤途径之一。
Objective：To study the effects of the ethanol extract from Ajuga decumbents on blood-activating and stasis-dissolving and anti-liver acute injury in mice.Method： Fifty mice were randomly divided into 5 groups：normal control group,aspirin group,ethanol extract from A.decumbents groups of low,middle and high doses.Treatments in according groups were given daily for 7 days.Glass capillary method and slide method were used to determinate the effects of the ethanol extraction of A.decumbens on blood-activating and stasis-dissolving by the clotting time in mice.Meanwhile we selected the intestinal mucosa to measure its nodal points of capillary network,the diameter of capillary and the blood＇s flow velocity.Another 60 mice were randomly divided into 6 groups：normal control group,model group,bifendete group,ethanol extract from A.decumbents groups of low,middle and high doses.The acute liver injury model in mice was induced by CCl4 or D-GalN.Treatments in according groups were given daily for 7 days.The protective effect for liver was investigated by detecting the levels of ALT,AST in serum.Result： The ethanol extract from A.decumbens could significantly prolong the clotting time（P0.05）,increase the blood flow velocity and the intestinal mucosa＇s nodal points of capillary network（P0.05） in normal mice.It could markedly decrease the level of ALT and AST in serum in the acute liver injured mice induced by CCl4 or D-GalN（P0.05）.Conclusion： The ethanol extract from A.decumbens has the effect of protecting liver.It may be related with effects of blood-activating and stasis-dissolving.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第17期209-212,共4页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
金疮小草
乙醇提取物
活血化瘀
急性肝损伤
小鼠
Ajuga decumbens
ethanol extraction
effects of blood-activating and stasis-dissolving
acute liver injury
mice
