摘要
目的:建立同时测定人血浆中舍曲林、喹硫平浓度的高效液相色谱法。方法:以Diamonsil TM C18反相柱(150 mm×4.6 mm,5μm)为色谱柱,流动相为30 mmol.L-1醋酸铵-乙腈(24∶76);流速1.0mL.min-1;柱温40°C;检测波长210 nm。以醋酸乙酯与二氯甲烷(80∶20)为提取剂。结果:舍曲林在20.0～480.0μg.L-1、喹硫平在25.0～1 000.0μg.L-1范围内,峰面积与其浓度呈良好线性关系;舍曲林、喹硫平的低、中、高3种浓度相对平均回收率大于95%,提取回收率大于70%。日内、日间RSD均低于10%(n=5)。结论:该方法灵敏、准确、简单、快速,可用于临床血药浓度监测和药动学研究。
OBJECTIVE To establlsh a method for determining the concentration of sertraline and guetiapine in human plasma by HPLC. METHODS The concentration of plasma drug was analyzed in a reverse phase HPLC system C18 column（ 150mm×4.6mm,5μm）： mobile phase consisted of 30 mmol·L^-1 ammonioum-acetonitrile （24：76）; the flow rate was 1.0mL·min^-1; the detection wavelength was at 210 nm. Ethyl acetate and dichloromethane（80：20） was used as extracting solvent. RESUETS The calibration curves showed good Linearity range of 20.0-480.0μg·L^-1 for sertraline, 25.0～1000.0μg·L^-1 for quetiapine respectively. The average recoveries of sertraline and quetiapine in low, middle and high concentrations were over 95% respectively% the extraction recovery over 70% respectively. The intra-day and inter-day variation（RSD）was less than 10% （n= 5）. The minimum detectable concentration of sertraline and quetiapine was 10. 0 μg·L^-1. CONCLUSION The method is sensitive, accurate, simple and quick, it can be used for clinical drug monitoring and pharmacokinetics studies.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2011年第17期1436-1439,共4页
Chinese Journal of Hospital Pharmacy
基金
广东省自然科学基金资助项目(编号:8151037001000001)
