摘要
通过ISSR技术对19个野生天门冬居群共67个个体进行遗传多样性分析。结果表明:用13个随机引物共扩增出125条清晰条带,其中92条具多态性,平均多态性位点比率为73%,建立了不同居群的ISSR标记;Nei’s基因多样性指数H=0.19,Shannon’s多样性指数I=0.30,遗传分化指数Gst=0.8206。聚类分析表明,青海、云南和贵州省的5个居群聚为一大支,其它居群为另一大支。野生天门冬种内具有较高的遗传多样性,遗传变异主要存在于居群间;ISSR标记可以作为研究天门冬遗传多样性及居群鉴定的有效标记。
The genetic diversity of 67 individuals from 19 populati-ons was analyzed by ISSR.The results showed that 13 primers were selected to produce highly reproducible ISSR bands,92 showed polymorphism among 125 amplified bands and the percentage of polymorphic bands reached 73%,ISSR marker of different populations was found.Nei＇ s gene diversity index（H） was 0.192,Shannon＇s information index was 0.30,genetic differentiation index was 0.8206.The Clustering tree showed the populations of Qinghai and Yunnan province,5 populations of Guizhou province were a branch,others populations were another branch.Asparagus had a high genetic diversity and the majority of genetic variation occurred among populations.The ISSR marker could be used for analysis of genetic diversity and identification of genetic variation of Asparagus cochinchinensis.
出处
《北方园艺》
CAS
北大核心
2011年第16期164-166,共3页
Northern Horticulture
基金
湖南省高校创新平台开放基金资助项目(09K106)
关键词
天门冬
居群
ISSR
遗传多样性
Asparagus cochinchinensis
populations
ISSR
genetic diversity
