摘要
背景:骨质疏松症是一种多基因遗传病,骨钙素受体基因多态性与骨密度关系存在地域和人群的差异。目的:观察绝经后妇女骨钙素基因型频率分布及其与骨密度的关系,探讨福州地区汉族绝经后妇女骨质疏松症的遗传易感基因。方法:用聚合酶链式反应限制性片段长度多态性分析201例汉族绝经后妇女骨钙素基因型,用双能X射线吸收法测定腰椎、股骨颈,大转子和Ward’s三角4个部位骨密度值。结果与结论:福州地区汉族绝经后妇女骨钙素基因型频率分布符合Hardy-Weinberg定律(χ2=2.29,P>0.05),基因多态性分布依次为HH5%、hh46%、Hh49%,与福州、北京、广州、台湾地区骨钙素基因HindⅢ位点多态性分布频率差异无显著性意义(P>0.05)。但是与日本人、白种人差异明显(P<0.05)。且HH基因型在大转子骨密度明显高于hh型(P<0.05),但不同基因型在第2～4腰椎、股骨颈、Ward’s三角区的骨密度差异无显著性意义。提示绝经后妇女骨钙素基因型与大转子骨密度可能存在一定关联。
BACKGROUND：Osteoporosis is a polygenic disease.The relationships between osteocalcin（OC） genotypes and bone mineral density（BMD） are different from regions and populations.OBJECTIVE：To investigate the relationship between OC genotypes and BMD in postmenopausal women,so as to explore the susceptibility gene of female postmenopausal osteoporosis in Fuzhou region.METHODS：The OC genotype was determined by polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP）.The BMD of lumbar spine,femoral neck,trochanter and Ward＇s triangle of 201 Han Chinese postmenopausal women were measured by dual energy X-ray absorptiometry.RESULTS AND CONCLUSION：The distribution of OC genotypes of Han Chinese postmenopausal women in Fuzhou followed the Hardy-Weinberg equilibrium（X^2=2.29,P〈0.05）.The frequencies of OC polymorphism were HH genotype 5%,hh 46%,and Hh 49%,which was not significant different from those of Fuzhou,Beijing,Guangzhou and Taiwan（P〈0.05）,but obviously different from Japanese and Caucasians（P〈0.05）.There was a significant difference in BMD of trochanter between HH and hh genotypes（P〈0.05）,but the difference was not significant in vertebrae L2-4,femoral neck and Ward＇s triangular area.There is some association between OC genotypes and BMD in postmenopausal women possibly.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2011年第15期2825-2828,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
2007年福建省教育厅资助项目(JA07128)
骨钙素基因Hind Ⅲ多态性对绝经后妇女骨密度的影响~~
