摘要
为获得优质的愈伤组织,以青钱柳嫩茎和叶片作为外植体诱导愈伤组织,研究了不同的消毒方法、基本培养基和植物生长调节剂对青钱柳叶片和茎段愈伤组织诱导的影响。结果表明,以青钱柳茎段为外植体诱导愈伤组织,最佳的消毒组合为70%酒精10 s+0.1%升汞4 min,污染和死亡率最低(18.0%),诱导率最高(95.0%)。以青钱柳叶片为外植体诱导愈伤组织,最佳的消毒组合为70%酒精0 s+0.1%升汞4 min,污染和死亡率最低(13.7%),诱导率最高(96.3%)。以青钱柳茎段、叶片为外植体诱导愈伤组织的最佳基本培养基分别为改良MS和MS,诱导率最高分别为95.56%和96.37%。激素组合KT0.5 mg.L-1+2,4-D2.0 mg.L-1效果最佳,诱导率高达97.5%。研究结果为青钱柳愈伤组织诱导最适培养条件的筛选提供依据,同时也为进一步建立青钱柳组培快繁及悬浮培养体系提供基础。
Stems and leaves of Cyclocarya palhtru were used as explants to induce callus in order to obtain better callus. Effects of different disinfection methods, basic culture media, and plant growth regallators on callus induction of C. pallium were studied. When stems of C. paliuruz were used as explants, the best disinfection combination was to treat the stems with 70% alcohol for 10 seconds and 0.1% mercuric chloride for 4 minutes, with the lowest contamination rate and mortality of 18.0% and the highest gelanination rate of 95.0%. When leaves of C. paliurus were used as explants, the best disinfection formulation was to treat the leaves with 70% alcohol for a very short time and 0. 1% mercuric chloride for 4 minutes, with the lowest contamination rate and mortality of 13.7% and the highest germination rate of 96.3%. The optimal media for callus induction of C. paliuru using stems and leaves as explants were modified MS and MS respectively, in which the highest induction rates were 95.56% and 96.37% respectively. The best combination of plant growth regulators was 0.5 mg· L^-1 KT plus 2.0 mg· L^-1 2,4-D, and the induction rate reached 97.5%.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2011年第9期8-10,共3页
Journal of Northeast Forestry University
基金
国家林业局林业公益性行业科研专项(200904046)
江苏省高等学校创新团队科研计划项目(苏教科[2009]10号)
江苏省"六大人才高峰"项目
关键词
青钱柳
叶片
茎段
愈伤组织
植物生长调节剂
诱导
Cyclocarya paliurus
Leaves
Stems
Callus
Plant growth regulators
Induction
