摘要
目的:研究复方金钱草颗粒对肾结石、利尿、解痉、抗炎作用,为其临床应用提供药理学依据。方法:选用雄性Wistar大鼠50只,体重(200±20)g,随机分为正常组、肾结石(模型组)、复方金钱草颗粒大、小剂量(7.4,1.8 g.kg-1)组、阳性药肾石通颗粒(3.3 g.kg-1)组。采用乙二醇和氯化铵诱导大鼠肾草酸钙结石模型,造模1周后开始给药,给药的同时继续给予造模剂,连续8周。8周后,观察大鼠成石率和检测各组大鼠血清尿素氮(BUN)、肌酐(Cr)浓度和肾组织Ca2+含量;离体输尿管试验观察复方金钱草颗粒对兔离体输尿管平滑肌的影响;代谢笼法观察复方金钱草颗粒的利尿作用;二甲苯致小鼠耳廓肿胀法和大鼠棉球肉芽组织增生法观察复方金钱草颗粒的抗炎作用。结果:复方金钱草颗粒7.4 g.kg-1剂量组能显著抑制大鼠的成石率,能显著提高肾结石大鼠肾脏系数,显著降低肾钙含量、血清BUN和Cr水平(P<0.05);能显著增加大鼠的水负荷尿量(P<0.05);显著减轻由Ach引起的兔离体输尿管痉挛(P<0.05);能显著减轻二甲苯致小鼠耳廓肿胀和棉球肉芽组织增生(P<0.05)。结论:复方金钱草颗粒具有抗肾结石,利尿,减轻输尿管张力和抗炎作用,对尿路结石有良好的防治作用。
Objective：To study the influence of complex herba lysmachiae gramules on the experimental kidney stone and its diuretic,spasmolyti and anti-inflammatory effects.Method：Fifty Wistar rats（n=10） were randomly divided into five group,the normal goups,the model group,the Shenshitong gramules group（3.3 g·kg-1）,high complex herba lysmachiae gramules group（7.4 g·kg-1） and low complex herba lysmachiae gramules group（1.8 g·kg-1）.The rat model of renal calcium oxalate stones was induced by administrated ethylene glycol and ammonia chloride,after 8 weeks modeled and administered,the incidence of kedney stone,calaium in kedneys,serum BUN,serum Cr were detected.The effects of complex herba lysmachiae gramules on ureter smooth muscle of rabbits in vitro,water-loaded diuretic in rats and anti-inflammatory was evaluated.Result：The number of renal stone formation,the contentration of calcum in kedneys,serum BUN and serum Cr were lower in high complex herba lysmachiae gramules group than that in model group,and lasted diuretic effects in water-loaded rat.High complex herba lysmachiae gramules group could obviously inhibit the average tension amplified on the ureter smooth muscle induced by Ach,and could markedly suppressed xylene-caused mouse ear swelling and granuloma by cotton ball.Conclusion ：Complex herba lysmachiae gramules could inhibit calcium oxalate stone formation in experimental rats,and had diuresis,spasmolytic effects in vitro and anti-inflammatory actions.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第18期206-209,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广西科学研究与技术开发计划项目(桂科攻1099062-4)
关键词
复方金钱草颗粒
肾结石
利尿
解痉
抗炎
complex herba lysmachiae gramules
experimental kidney stone
diuresis
spasmolysis
anti-inflammatory
