摘要
采用超声辅助提取苦瓜中的活性成分,利用分散液液微萃取(DLLME)技术对提取液进行浓缩,设计正交试验优化DLLME条件。在优化条件下,对不同产地苦瓜的超声提取液进行微萃取,萃取液进行HPLC分析,确定6个共有峰作为苦瓜的指纹峰,采用《中药色谱指纹图谱相似度评价系统》2004A版评价指纹谱图,不同产地苦瓜的HPLC指纹谱图与对照谱图(长沙苦瓜)之间的相似度均大于0.960。本研究为天然产物的鉴定和质量控制提供了一个新的途径。
Active ingredients in Momordica charantia were extracted with ultrasonic assistance.The extracts were concentrated by dispersive liquid-liquid microextraction（DLLME） technique.Orthogonal experimental design was adopted to optimize DLLME conditions.Under optimum conditions,the extracts of Momordica charantia from different areas were microextracted,and then were analyzed by high performance liquid chromatography（HPLC）.Six common peaks were identified as fingerprint peak.The HPLC fingerprint peaks were assessed by Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine（Version 2004A）.The similarity of the fingerprint of Momordica charantia from different areas was greater than 0.960 compared with control group from Changsha.A novel approach for characterization and quality control of natural products was provided.
出处
《广州化工》
CAS
2011年第17期8-10,共3页
GuangZhou Chemical Industry
基金
国家自然科学基金项目(20905045)
河南省科技厅科技攻关项目(102102310385)
河南省教育厅自然科学基础研究计划项目(2009B150021)
商丘师范学院青年科学基金项目(2009QN09)资助
关键词
苦瓜
超声
分散液液微萃取
高效液相色谱
指纹图谱
Momordica charantia
ultrasonic assistance
dispersive liquid-liquid microextraction
HPLC
fingerprint
