摘要
目的:建立河北道地药材北沙参HPLC-PDA指纹图谱分析方法,并与不同产地北沙参药材指纹图谱特征进行比较,为科学评价与有效控制北沙参药材质量提供新方法。方法:采用DiamonsilTM C18(250 mm×4.6 mm,5"m)色谱柱,流动相为甲醇-乙腈-0.05%磷酸水溶液,梯度洗脱,流速1.0 mL.min-1,柱温30℃,检测波长295 nm。结果:13批道地药材的相似度在0.9以上,化学组成一致性较好;不同产地饮片相似度只在0.1～0.4之间。结论:本方法操作简便、快速、准确,为北沙参药材的鉴别和质量的全面控制提供了依据。
Objective：To establish a fingerprint analysis method for identification of Radix Glehniae of Hebei province,get the control chromatogram and compare the fingerprints of Radix Glehniae collected from different producing areas so as to establish a sensitive and specific method for controlling the quality of Radix Glehniae.Methods：Chromatographic separation was carried out on DiamonsilTM C18（250 mm×4.6 mm,5 μm） column at 30 ℃.The mobile phase was methanol,acetonitrile and phosphoric acid in aqueous solution（0.05%） with gradient elution.The flow rate of mobile phase was maintained at 1.0 mL·min-1.The detection wavelength was set at 295 nm.Results：The similarity between 13 batches famous-region drugs were above 0.9,and the chemical composition of them were good consistency.The similarity between other samples from different sources were only 0.1-0.4.Conclusion：This method of the operation is simple,quick,accurate,and can be used for the identification and quality control of Radix Glehniae.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2011年第9期1683-1688,共6页
Chinese Journal of Pharmaceutical Analysis
基金
河北省科技支撑计划项目(课题号:09276423D)
关键词
中药
伞形科
珊瑚菜
北沙参
饮片
补骨脂素
花椒毒素
佛手柑内酯
欧前胡素
异欧前胡素
呋喃香豆素
高效液相色谱
指纹图谱
特征图谱
traditional Chinese medicine
Umbelliferae
Glehnia littoralis
Radix Glehniae
prepared drug in pieces
psoralen
xanthotoxin
bergapten
imperatorin
isoimperatorin
furocoumarin
HPLC
fingerprint
specific chromatogram
