摘要
目的:建立同时测定藿香正气胶囊中甘草苷、柚皮苷、橙皮苷、麝香草酚、欧前胡素、和厚朴酚、异欧前胡素及厚朴酚共8个主要化学成分的HPLC分析方法。方法:采用C18(250 mm×4.6 mm,5μm)色谱柱,流动相为水-甲醇-乙腈,梯度洗脱(0.01～20 min,68∶30∶2→60∶38∶2;20～50 min,60∶38∶2→34∶64∶2;50～65 min,34∶64∶2;65～75 min,34∶64∶2→28∶70∶2;75～85 min,28∶70∶2→68∶30∶2),流速1.0 mL.min-1,检测波长283 nm。结果:在各自的线性范围内甘草苷、柚皮苷、橙皮苷、麝香草酚、欧前胡素、和厚朴酚、异欧前胡素、厚朴酚8个化合物的标准曲线呈良好的线性关系;平均加样回收率分别为97.4%,98.5%,97.4%,98.6%,97.8%,99.2%,97.0%,97.5%;所有化合物的精密度和重复性试验的RSD均<3.0%。结论:该分析方法简单、灵敏、准确,重复性好,可同时分析藿香正气胶囊中的主要成分。
Objective：To establish an HPLC method for determination of eight main components（liquiritin,naringin,hesperidin,thymol,imperatorin,honokiol,isoimperatorin,and magnolol） in the traditional Chinese medicinal preparation Huoxiang Zhengqi capsules.Methods：The separation was performed on a C18（250 mm×4.6 mm,5 μm） column by gradient elution with water–methanol–acetonitrile（0.01-20 min,68∶30∶2→60∶38∶2;20-50 min,60∶38∶2→34∶64∶2;50-65 min,34∶64∶2;65-75 min,34∶64∶2→28∶70∶2;75-85 min,28∶70∶2→68∶30∶2） as the mobile phase at a flow rate of 1 mL·min-1,with UV detection at 283 nm.Results：Eight regression equations showed good linear relationships between the peak area ratio of each marker to internal standard and amounts.The recoveries of the markers listed above were 97.4%,98.5%,97.4%,98.6%,97.8%,99.2%,97.0%,and 97.5%,respectively.The RSDs of precision and repeatability test were less than 3.0%.Conclusion：The method is simple,sensitive and reliable.It can be used for quantitative determination of Huoxiang Zhengqi capsules.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2011年第9期1776-1780,共5页
Chinese Journal of Pharmaceutical Analysis
基金
黔科合NY字[2009]3021
黔省专合字(2010)74
"西部之光"人才项目
黔科合院所创新[2010]4010-1
黔科院J合字[2010]001号
