摘要
以烤烟台烟7号与白肋烟白肋21作为杂交亲本,后代自交衍生的127个F2和F2:3家系为材料,构建了全长为3483cM的包括26个连锁群、190个标记位点的烟草遗传连锁图谱。通过一年两地各3次重复的随机区组田间试验,测定烟叶烟碱、总氯、总钾、叶长、茎叶夹角、白粉病6个重要性状,采用混合线性模型的复合区间作图法定位QTL并分析其遗传互作效应。结果检测到2个烟碱相关QTL、2个总氯相关QTL、1个总钾相关QTL、4个叶长相关QTL、茎叶夹角和白粉病相关QTL各1个,其中6个加性效应QTL和4对加加上位性效应QTL。这11个基因位点遗传效应中除加性效应外,上位性效应也具有重要作用。
The agronomic traits,chemical components,and diseases are very important factors affecting the yield and leaf quality of tobacco.QTLs linked to the three aspects of traits can be used in marker-assisted selection for the yield and leaf quality improvement in tobacco.The objective of this study was to identify QTLs controlling important traits in tobacco,which could promote the basic research of molecular marker-assisted selection breeding.A population with 127 F2 and F2：3 individuals from the cross between Taiyan 7 and Burley 21 was used to establish a genetic linkage map of tobacco,190 marker loci were mapped into 26 linkage groups which spanned a total map length of 3 483 cM.With a randomized complete block design,two location field tests with three replicates one year were conducted to charactererize six important traits,including nicotine,total chlorine,total kalium,leaf length,leaf angle,and powdery mildew.Utilizing data of field trials and MCIM method,QTLs for six important characters were mapped and their genetic interactions were analyzed.As the results,two QTLs for nicotine,two QTLs for total chlorine,one QTL for total kalium,four QTLs for leaf length,one QTL for leaf angle,and one QTL for powdery mildew were detected,of which six QTLs had additive effect,and the others were epistatic.The results indicated that epistatic QTLs,the additive QTLs also played important roles in the genetic basis of the six important traits with eleven gene loci in tobacco.
出处
《作物学报》
CAS
CSCD
北大核心
2011年第9期1577-1584,共8页
Acta Agronomica Sinica
基金
国家烟草专卖局科技项目(110200601006)
国家烟草专卖局资助项目(国烟科[2005])资助
关键词
烟草
性状
QTL定位
上位性
Tobacco （Nicotiana tobacum L.）
Trait
QTL
Epistasis
