摘要
目的:探讨复方大青叶注射液对体外培养HT-H9细胞中CXCR4启动子活性的作用。方法:克隆人CX-CR4启动子序列,构建报告载体pGL4.17-CXCR4;转染HT-H9细胞株,G418筛选后分组,高、低剂量实验组分别用200、100mg/L大青液注射液处理,设注射水对照组、空白对照组和未转染对照组;各组处理24h后,进行荧光素酶活性检测。结果:成功构建含人CXCR4启动子序列的报告载体PGL4.17-CXCR4;5组荧光素酶活性比较差异有统计学意义(F=135.510,P<0.001)。复方大青叶注射液高剂量组与低剂量组荧光素酶活性均明显低于对照组(P<0.01)。结论:复方大青叶注射液能够有效抑制体外培养HT-H9细胞中CXCR4启动子活性。
Aim：To investigate the effect of the compound Folium Isatidis injection on the activity of CXCR4 promoters in HT-H9 cells cultivated in vitro.Methods：The sequence of CXCR4 promoter gene was cloned,and inserted into the reporter vector pGL4.17 to construct luciferase reporter vector （pGL4.17-CXCR4）,the recombinant plasmids were transfected into HT-H9 cells.Screened by G418,the lasting transfected cells were allocated into 4 groups： high dose group and low dose group （treated with 200,100 mg/L compound Folium Isaticlis injection for 24 h,respectively）,injection water control group,blank control group,and untransfection group.The luciferase activity was evaluated.Results：The results of restrict double digestions and DNA sequencing proved that pGL4.17-CXCR4 was constructed successfully.The level of luciferase activity among the 5 groups had significant differences （F=135.510,P〈0.001）.The levels of the compound Folium Isatidis injection groups,both high dose group and low dose group,were remarkably lower than those of control groups （P〈0.01）.Conclusion： The compound Folium isatidis injection could obviously restrain the activity of CXCR4 promoters in HT-H9 cells in vitro.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2011年第5期681-684,共4页
Journal of Zhengzhou University(Medical Sciences)
基金
河南省医学科技攻关基金资助项目2008002002
