摘要
目的:通过正交试验设计法优选甘蔗叶粗多糖的提取工艺条件。方法:采用L9(34)正交试验设计法优化甘蔗叶提取工艺,通过分光光度法,以苯酚-硫酸法测定粗多糖含量,以多糖提取量为指标优选甘蔗叶粗多糖最佳提取工艺。结果:甘蔗叶粗多糖提取的最佳条件为料液比1∶30,于100℃提取3次,每次5 h。结论:验证试验表明优选出的提取工艺稳定、合理、可行。
Objective：To optimize the extraction process of polysaccharide from sugarcane leaves by orthogonal design.Method：The yield of polysaccharide as index to optimize extraction process of polysaccharide from sugarcane leaves by orthogonal test with spectrophotometry.The content of polysaccharide was determined by phenol-sulfuric acid method.Result：The optimum extraction technology was as follows：extracted 3 times at 100 ℃ with 30 times amount of deionized water for 5 h per time.Conclusion：The verification test showed that the optimum extraction process was stable,reasonable and feasible.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第19期11-13,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
2007年第二批重点实验室能力提升及培育基地建设项目基金(桂科能07109004-02k)
广西壮族自治区中医药管理局中医药科技专项课题(GZKZ09-9)
2009广西教育厅面上项目(200911MS125)
广西技术研究与开发项目(桂科攻10124008-11)
关键词
甘蔗叶
多糖
正交试验
sugarcane leaves
polysaccharide
orthogonal test
