摘要
目的观察17β-雌二醇对豚鼠胆囊平滑肌的基因组和非基因组效应，初步探究其机制。方法构建去卵巢豚鼠动物模型，皮下注射17β-雌二醇，检测各组豚鼠血清雌二醇和八肽胆囊收缩素（CCK-8）含量；采用张力换能器观察17β-雌二醇对胆囊离体肌条收缩的影响。观察17β-雌二醇对豚鼠胆囊平滑肌肌条的急性效应及可能机制。结果与假手术组相比，去卵巢组豚鼠血清雌二醇和CCK-8含量降低（P〈0．05），离体胆囊肌条对CCK-8以及乙酰胆碱的敏感性升高（P〈0．05）。随着皮下注射17β-雌二醇时间（1、3和7d）的延长，二者含量升高（P〈0．05），离体胆囊肌条对CCK-8和乙酰胆碱的敏感性下降（P〈0．05）。10^-9～10^-7mol／L的17β-雌二醇对正常豚鼠离体胆囊肌条收缩无明显影响（P〉0．05），10^-6～10^-5mol／L的17β-雌二醇可以抑制胆囊肌条收缩（P〈0．05），这种抑制效应可以被尼莫地平、阿托品、地伐西匹、ICI182，780和Y-27632等阻断剂阻断。结论17β-雌二醇与胆囊动力相关，可以通过基因组和非基因组机制影响胆囊平滑肌的收缩。
Objective To investigate the genomic and non-genomic effects of 17β-estradiol on gallbladder smooth muscle strips of guinea pig and their possible mechanism. Methods After ovariectomized operation （OVX） and subcutaneous injection of 17β-estradiol, the contents of serum estradiol and cholecystokinin-octopeptide （CCK-8） of the sham operation group （Sham） guinea pigs, the OVX group, the OVX and subcutaneous injection 17β-estradiol for 1 day （OVX＋E2, 1 d）, 3 days （OVX＋ E2, 3 d） and 7 days （OVX＋E2, 7 d） were detected by EILSA respectively. The effects of CCK-8/ Ach on constriction of gallbladder muscle strip were observed among various groups by using tension transducer, and the acute effects of 17β-estradiol on guinea pig gallbladder smooth muscle strips were observed to probe its possible mechanism. Results Compared with the Sham group, the serum contents of estradiol and CCK-8 decreased in OVX group （P〈0. 05） whereas the sensitivity of OVX guinea pigs gallbladder muscle strips to CCK-8/Ach increased （P〈0.05）. With the extension of the subcutaneous injection 17β-estradio1 time （for 1, 3, 7 days）, both the serum estradiol and CCK level increased （P〈 0. 05） while the guinea pig gallbladder strips sensitivity to CCK-8/Ach decreased （P〈0.05）. 17β-estradiol at concentration ranging from 10 9 to 10 7 mol/L have no effect on the guinea pig gallbladder strips contraction （P〉0.05）, but at concentration of 10 6 and 10 s mol/L, it can inhibit the gallbladder contraction （P〈0.05）. The blocking agents, such as nimodipine, atropine, devazepide, ICI 182,780 and Y-27632, can block the inhibited effects of 17β-estradiol. Conclusion The 17β-estradiol can affect the gallbladder motility, both by genomic and non-genomic pathway.
出处
《中华肝胆外科杂志》
CAS
CSCD
北大核心
2011年第9期771-774,共4页
Chinese Journal of Hepatobiliary Surgery
关键词
17Β-雌二醇
平滑肌
胆囊
17β-estradiol
Smooth muscle
Contraction
Gallbladder
