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应用IMS-RT-PCR方法检测番茄黑环病毒 被引量:4

Detection of Tomato Black Ring Virus by Immunomagnetic Separation and RT-PCR
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摘要 以番茄黑环病毒为研究对象,用免疫磁珠分离(Immunomagnetic separation,IMS)结合RT-PCR方法,建立了检测番茄黑环病毒的IMS-RT-PCR方法。结果表明,该方法能够检测到较低浓度的病毒,灵敏度比普通RT-PCR的灵敏度高出10倍。 A high sensitivity method for detecting Tomato Black Ring Virus (TBRV)was developed by immunomagnetic separation and reverse transcriptase-polymerase chain reaction(IMS-RT-PCR). Results showed that the, sensitivity of IMS-RT-PCR for detecting TBRV was ten times higher than that of the traditional RT-PCR.
出处 《上海交通大学学报(农业科学版)》 2011年第4期21-24,37,共5页 Journal of Shanghai Jiaotong University(Agricultural Science)
基金 上海市技术性贸易措施应对专项项目(09TBT002)
关键词 IMS-RT-PCR 番茄黑环病毒 检测 IMS-RT-PCR Tomato Black Ring Virus detection
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参考文献6

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  • 4Scott J Harper,Catia Delmiglio,Lisa I Ward,et al. Detection of Tomato black ring virus by real-time onestep RT-PCR [J ]. Journal of Virological Methods, 2011,171 : 190-194.
  • 5杨翠云,于翠,宋绍祎,陶庭典.番茄黑环病毒分子生物学检测方法及分离物序列分析[J].植物检疫,2006,20(5):275-278. 被引量:10
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