摘要
以番茄黑环病毒为研究对象,用免疫磁珠分离(Immunomagnetic separation,IMS)结合RT-PCR方法,建立了检测番茄黑环病毒的IMS-RT-PCR方法。结果表明,该方法能够检测到较低浓度的病毒,灵敏度比普通RT-PCR的灵敏度高出10倍。
A high sensitivity method for detecting Tomato Black Ring Virus (TBRV)was developed by immunomagnetic separation and reverse transcriptase-polymerase chain reaction(IMS-RT-PCR). Results showed that the, sensitivity of IMS-RT-PCR for detecting TBRV was ten times higher than that of the traditional RT-PCR.
出处
《上海交通大学学报(农业科学版)》
2011年第4期21-24,37,共5页
Journal of Shanghai Jiaotong University(Agricultural Science)
基金
上海市技术性贸易措施应对专项项目(09TBT002)