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葛根素口服微乳在Caco-2细胞模型中摄取特性研究

Study on Intestinal Absorption of Puerarin Microemulsion Using Caco-2 Cell Model
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摘要 目的研究葛根素口服微乳在Caco-2细胞模型中的吸收特性,为进一步探讨葛根素微乳的吸收机制提供依据。方法利用MTT实验筛选出葛根素在Caco-2细胞中的安全浓度,考察时间、pH值及药物浓度对葛根素微乳吸收的影响。结果葛根素微乳在Caco-2细胞模型的摄取实验中,随着葛根素微乳浓度的增加,葛根素吸收增加。结论葛根素微乳在Caco-2细胞模型中的主要吸收机制可能是被动转运。 OBJECTIVE To investigate the absorption of puerarin microenmlsion using Caco-2 （：ell model and explore the mecha- nism of its absorption. METHODS The safety concentration of puerarin in Caco-2 cell was first selected by MTI＂ method. Then the effects of time,pH and durg concentration on the absorption were investigated. RESULTS With the increase of the concentration of puerarin microemnlsion, the absorption of puerarin increased. CONCLUSION The absorption of puerarin inicroemulsion in Caco-2 cell model is maybe through a passive transcellular transport.
作者 胡雄彬 唐甜甜 邓长凤 向大位 向大雄
机构地区 中南大学湘雅二医院湖南省中药制剂新技术重点研究室 中南大学药学院
出处 《中国药学杂志》 CAS CSCD 北大核心 2011年第19期1506-1509,共4页 Chinese Pharmaceutical Journal
基金 湖南省自然科学基金资助项目(08JJ3053)
关键词 葛根素 微乳 CACO-2细胞模型 被动转运 puerarin microemulsion Caco-2 cell model passive transport
分类号 R944 [医药卫生—药剂学]
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参考文献6

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二级参考文献8

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共引文献33

中国药学杂志
2011年 第19期

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