摘要
目的探讨坐骨海绵体肌损伤性勃起功能障碍的修复治疗效果。方法选用生殖期雄性Wistar大鼠150只,采用抓阄法随机分为修复组1(n=30)、修复组2(n=30)、修复组3(n=30)、修复组4(n=30)、未修复组(n=10)、假手术组(n=10)及正常组(n=10)。修复组1切断双侧坐骨海绵体肌后立即将其缝合,修复组2、3、4分别在切断双侧坐骨海绵体肌后2、4、8周重新缝合切断的双侧坐骨海绵体肌,未修复组切断双侧坐骨海绵体肌后不作修复,正常组不做手术及修复处理。假手术组模拟手术操作过程,但不切断双侧坐骨海绵体肌。选用育龄期未孕雌性Wistar大鼠150只,采用抓阄法随机与上述各组一一配对饲养,观察2～4个月,了解各组雌鼠受孕情况。通过雌鼠受孕间接推断其配对雄鼠勃起功能恢复。结果修复组1雄鼠死亡3只,有效配对雌鼠27只,17只受孕,受孕率63.0%;修复组2雄鼠死亡6只,有效配对雌鼠26只,16只受孕,受孕率61.5%;修复组3雄鼠死亡5只,有效配对雌鼠26只,15只受孕,受孕率57.7%;修复组4雄鼠死亡9只,有效配对雌鼠23只,14只受孕,受孕率60.9%;未修复组雄鼠死亡1只,有效配对雌鼠9只,均未受孕;正常组与假手术组无死亡,配对雌鼠10只,均受孕。正常组与假手术组雌鼠受孕情况比较差异无统计学意义(P>0.05),正常组与未修复组及各修复组比较差异有统计学意义(P<0.05),假手术组与未修复组及各修复组比较差异有统计学意义(P<0.05),未修复组与各修复组比较差异有统计学意义(P<0.05),而各修复组之间两两比较差异无统计学意义(P>0.05)。结论修复切断的雄鼠双侧坐骨海绵体肌能改善勃起功能障碍,但与修复时间选择关系不大。
Objective To explore the efficacy of repair treatment on rat erectile dysfunction induced by ischiocavernosus trauma.Methods One hundred and fifty male Wistar rats were randomly divided into a repair group Ⅰ（n=30）,a repair group Ⅱ（n=30）,a repair group Ⅲ（n=30）,a repair group Ⅳ（n=30）,a non-repair group（n=10）,a sham operation group（n=10） and a normal group（n=10）.Both sides of rat ischiocavernosus were sewed up at different time of immediately,2 weeks,4 weeks and 8 weeks after the rat ischiocavernosus were severed in the four repair groups.The severed rat ischiocavernosus were not repaired in the non-repair group.The rat ischiocavernosus in the normal group were neither severed nor sewed up.A similar operation was performed in the sham operation group,but rat ischiocavernosus were not severed.After the repair operation,150 female Wistar rats at reproductive age but not pregnant were randomly selected to match those male rats one by one,and the male erection recovery were indirectly deduced based on the female pregnancy rate after 2-4 month observations.Results In repair groups Ⅰ-Ⅳ： the number of dead male rats was 3,6,5 and 0,respectively;the number of successfully matched female rats was 27,26,26 and 23,respectively;the number of pregnant female rats and the pregnancy rate were 17（63.0%）,16（61.5%）,15（57.7%） and 14（60.9%）,respectively.In the non-repair group： 1 male rat died,and 9 female rats were successfully matched with a 0% pregnancy rate.In the normal group and the sham operation group： no rats died and ten female rats were successfully matched with a 100% pregnancy rate.The comparisons of the female rats conception between each group showed that： the difference between the normal group and the sham operation group was not statistically significant（P0.05）;both of the normal group and the sham operation group were statistically significant as compared with the non-repair group and the repair groups（P0.05）;the non-repair group was statistically significant as compared with each repair group（P0.05）;but the comparisons within the repair groups were not statistically significant.Conclusion The repair treatment of severed rat ischiocavernosus can improve rat erectile dysfunction,but the improvement is independent of the time.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2011年第19期2037-2039,共3页
Journal of Third Military Medical University
