摘要
为研究不同能量水平日粮对文昌鸡开产性状和开产前后(15、20和25周龄)NPY基因在下丘脑和卵巢组织中表达的影响,根据文昌鸡各生长阶段能量需要量的95%、100%、105%分别设置为低(Ⅰ)、中(Ⅱ)、高(Ⅲ)3个能量组进行饲养,每组5个重复,每个重复40只,并准确记录开产日龄、开产体重和开产蛋重,同时运用RT-PCR方法分别分析各能量组鸡只在开产前后下丘脑和卵巢NPY基因mRNA的表达量。结果表明,高能促进文昌鸡开产日龄提前;15、20和25周龄,低能组NPYmRNA表达量均高于中能组和高能组;15、20和25周龄低、中、高能组卵巢中NPYmRNA表达量变化不大,无显著差异。结果提示,能量水平的改变能够调节文昌鸡下丘脑和卵巢NPY基因mRNA表达和性成熟时间。
Objective to investigate the effect of different energy level diets on NPY mRNA expression level in hypothalamus and ovary at the time before and after laying（the age of 15 weeks,20 weeks and 25 weeks） and the traits in the first laying.In this research,600 Wenchang chickens were randomly divided into 3 groups： low energy group（GroupⅠ）,control energy group（GroupⅡ） and high energy group（Group Ⅲ）.There are 5 replications in each group,and 40 chickens in each replication.Subsequently,the age,the body weight and the egg weight in the first laying were recorded accurately.The NPY mRNA expression in hypothalamus and ovary in each energy groups before and after laying were analyzed by RT-PCR.The result showed that the high energy can advance the age of first laying.At the age of 15 weeks,20 weeks and 25 weeks,the NPY mRNA expression level of hypothalamus in low energy group was higher than that in the control and high energy groups,whereas,the NPY mRNA expression level of ovary among the three energy groups had no significant difference.The results indicated that the change of energy level was able to affect the NPY mRNA expression level in hypothalamus and ovary and the sex mature time of Wenchang chicken.
出处
《四川农业大学学报》
CSCD
北大核心
2011年第3期419-423,共5页
Journal of Sichuan Agricultural University
基金
国家高技术研究发展计划(2011AA100301)
农业行业专项研究计划(nyhyzx07-039)
现代农业产业技术体系建设专项资金
