摘要
为了研究饲料脂肪水平对吉富罗非鱼(Oreochromis niloticus)血液脂肪代谢的影响,选择均重为(2.63±0.16)g的健康的315尾吉富罗非鱼,随机分成3组,每组3个重复,投喂3组不同脂肪水平的等氮低脂肪组、中脂肪组、高脂肪组饲料(饲料中脂肪含量分别为3.71%、7.67%和16.55%),饲养90 d。禁食48 h再投喂,摄食不同脂肪饲料后0、6、12、24、48 h取样测定试验鱼血清中甘油三酯、胆固醇及血糖含量。结果显示:在48 h内,吉富罗非鱼血液中甘油三酯、胆固醇、血糖均呈现先上升后下降的趋势,在摄食后同一时间点,摄食高脂肪试验鱼血液甘油三酯和胆固醇显著高于低脂肪组(P<0.05),而血糖无显著变化,但有下降的趋势。结果说明:摄食后48 h内,吉富罗非鱼摄食低、中脂肪水平饲料后血液甘油三酯、胆固醇、血糖呈现单峰波变化规律,高脂肪诱导血液甘油三酯合成代谢,但没有改变其变化基本规律。
An experiment was carried out to evaluate the effects of dietary lipid levels on the blood lipometabolism parameters.Triplicate groups of fish（average weight ± SE,2.63±0.16 g） were fed three isonitrogenous experiment diets（30.40% crude protein,dry matter） formulated with increasing lipid levels（3.71%、7.67% and 16.55% lipid） using fish oil as the lipid source for 90 days.The blood was randomly collected from three fish in 3.71%,7.67% and 16.55% group at 0 h,6 h,12 h,24 h,48 h after the refeeding following the 48 h of fasting and the blood indices related to lipometabolism,i.e.blood sugar,triglyceride（TG）,cholesterol（CHO）,were detected.The results showed that after refeeding for 48 h,the TG,CHO and GLU were first increased and then decreased.At the same time-points after refeeding,the TG and CHO of the high-lipid group were significantly higher than that of the low-lipid group（P0.05）,the GLU had a decreasing trend but no significant change was observed（P0.05）.Those results indicated that the TG,CHO,GLU appeared to be a singlet curve and that the high-lipid dietary could reduce the metabolism of TG.
出处
《盐城工学院学报（自然科学版）》
CAS
2011年第3期9-14,共6页
Journal of Yancheng Institute of Technology:Natural Science Edition
基金
农业部水生动物遗传育种和养殖生物学重点开放实验室重点开放课题(BZ2007-06)
江苏省高校自然科学基础研究项目(08KJD240003)
江苏省"青蓝工程"优秀青年骨干教师培养基金项目[苏教师(2008)30号]
关键词
高脂肪
摄食
血脂
吉富罗非鱼
High lipid
Ingestion
Blood lipid
GIFT Nile tilapia（Oreochromis niloticus）
