摘要
目的建立HPLC测定桂利嗪胶囊含量的方法。方法采用Kromasil C18(4.6 mm×250 mm,5μl)色谱柱,流动相为甲醇-0.2%三乙醇胺-三乙胺(85∶15∶0.03),检测波长254 nm;柱温30℃;流速1 ml.min-1;样品处理方法:精密称取装量差异下的内容物(相当于桂利嗪25 mg),置25 ml量瓶中,加甲醇至刻度,滤过,再精密量取续滤液5 ml置50 ml量瓶中,摇匀,用0.45μm微孔滤膜滤过,取续滤液,即得。结果桂利嗪在19.79～197.94 mg.L-1的浓度范围内与峰面积呈良好的线性关系,r=0.999 9,平均回收率99.5%,RSD为0.6%(n=9)。结论该方法准确、重现性好,可作为该制剂的含量测定方法。
Aim To eatablish a method for determining the content of Cinnarizine Capsules.Method The HPLC analysis was performed on Kromasil C18 column（4.6 mm×250 mm,5 μl） with mobile phase of CH3OH-0.2triethanolamine-triethylamine（v：v：v=85 ： 15 ：0.03）,the flow rate was1.0 mL·min-1,the detection wavelength was at 254nm and the column temperature was 30℃.Results The linear range of Cinnarizine was 19.79~197.94 mg·L-1,and the average recovery was 99.5% with RSD being 0.6（n=9）% Conclusion This method is accurate,reliable and appropriate to control the quality of Cinnarizine Capsules.
出处
《安徽医药》
CAS
2011年第11期1362-1363,共2页
Anhui Medical and Pharmaceutical Journal
