摘要
目的探讨miR-18a和miR-18b在结肠癌中的表达及其临床意义。方法选取手术切除的原发性结肠癌标本20例,采用实时荧光定量PCR分析miR-18a和miR-18b的表达。结果相对于癌旁组织,20例结肠癌组织中miR-18a表达下调(P<0.05);其表达与TNM分期及浸润深度有关(P<0.05)。而miR-18b在癌组织中的表达则上调(P<0.05),其表达与TNM分期、浸润深度和远处转移无明显关联。结论 miR-18a可能参与了结肠癌的发展。
Objective To investigate the expression and clinical significance of miR-18a and miR-18b in colorectal cancer（CRC）.Methods Quantitative real time-PCR was applied to examine the expressions of miR-18a and miR-18b in 20 primary CRC specimens and tumor-adjacent tissues.The relationship between the expressions of miRNAs and clinicopathological features was analyzed.ResultsCompared to the tumor-adjacent tissues,the expression of miR-18a was down-regulated,but that of miR-18b was up-regulated in CRC（P0.05）.The expression of miR-18a in CRC was related to TNM stage and local invasion（P0.05）,which of miR-18b was not related to TNM stage,local invasion and metastasis.Conclusion The over-expression of miR-18a may play a role in the progression of CRC.
出处
《江苏医药》
CAS
CSCD
北大核心
2011年第19期2284-2286,共3页
Jiangsu Medical Journal
